Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	79,341	C→A	G38V (GGT→GTT)	leuD ←	3‑isopropylmalate dehydratase subunit LeuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	79,341	0	C	A	100.0%	132.2 / NA	37	G38V (GGT→GTT)	leuD	3‑isopropylmalate dehydratase subunit LeuD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/17);  total (20/17)

TTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAACCCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGC  >  NC_000913/79263‑79412                                                                               |                                                                        ttCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAacc                                                                       <  1:19376/81‑1 (MQ=255)  tCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCg                                                                      >  2:19416/1‑81 (MQ=255)    aGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTa                                                                    <  1:19377/81‑1 (MQ=255)     gCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTAc                                                                   <  2:19379/81‑1 (MQ=255)     gCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTAc                                                                   <  2:19378/81‑1 (MQ=255)         gAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACTGGt                                                               >  2:19412/1‑81 (MQ=255)         gAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGt                                                               >  1:19415/1‑81 (MQ=255)          aaGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGt                                                               >  2:19414/1‑80 (MQ=255)          aaGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTc                                                              <  1:19381/81‑1 (MQ=255)          aaGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTc                                                              <  2:19380/81‑1 (MQ=255)                  gTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTg                                                      <  1:19382/81‑1 (MQ=255)                        cTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCaaaaa                                                <  1:19383/81‑1 (MQ=255)                           ttGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACt                                              <  1:19384/80‑1 (MQ=255)                           ttGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTg                                             <  1:19385/81‑1 (MQ=255)                                      cATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGgatga                                  >  2:19419/1‑81 (MQ=255)                                      cATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGgatg                                   <  1:19386/80‑1 (MQ=255)                                        tCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGAtt                                >  2:19403/1‑81 (MQ=255)                                          cAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGc                              <  2:19387/81‑1 (MQ=255)                                            gAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCAt                            <  1:19388/81‑1 (MQ=255)                                               aaCGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCgg                         <  2:19389/81‑1 (MQ=255)                                               aaCGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCgg                         >  1:19420/1‑81 (MQ=255)                                                aCGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCgg                         >  2:19422/1‑80 (MQ=255)                                                  gCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTAt                      <  2:19390/81‑1 (MQ=255)                                                  gCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTAt                      >  1:19426/1‑81 (MQ=255)                                                   ccAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCCTCGGTATc                     >  1:19430/1‑81 (MQ=255)                                                   ccAGTCGTTAAACAGATGCGCGCCAAAAACCATACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATc                     <  2:19391/81‑1 (MQ=255)                                                     aGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGa                   >  2:19417/1‑81 (MQ=255)                                                     aGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGa                   <  1:19392/81‑1 (MQ=255)                                                             aaCAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTggcg           >  1:19434/1‑81 (MQ=255)                                                             aaCAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTggcg           >  1:19431/1‑81 (MQ=255)                                                              aCAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTggcgg          >  2:19440/1‑81 (MQ=255)                                                                aGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCa        >  2:19442/1‑81 (MQ=255)                                                                 gATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCAt       >  1:19423/1‑81 (MQ=255)                                                                  aTGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATc      >  1:19433/1‑81 (MQ=255)                                                                    gcgcgcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATc      >  1:19428/1‑79 (MQ=255)                                                                     cgcgcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAg   >  1:19456/1‑81 (MQ=255)                                                                      gcgcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGc  >  1:19446/1‑81 (MQ=255)                                                                      gcgcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGc  <  1:19393/81‑1 (MQ=255)                                                                               |                                                                        TTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAACCCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGC  >  NC_000913/79263‑79412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑