Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	930,073	G→A	A297V (GCG→GTG)	cydD ←	glutathione/L‑cysteine ABC exporter subunit CydD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	930,073	0	G	A	100.0%	103.1 / NA	29	A297V (GCG→GTG)	cydD	glutathione/L‑cysteine ABC exporter subunit CydD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/14);  total (15/14)

GTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCGCAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCGGTATCGTAGTGACCAAAATCCAGCTCGCCGAGATAG  >  NC_000913/929997‑930150                                                                             |                                                                              gTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAg                                                                           >  2:192965/1‑81 (MQ=255) gTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAg                                                                           >  1:192948/1‑81 (MQ=255)    aGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGAt                                                                        >  2:192954/1‑81 (MQ=255)    aGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGAt                                                                        >  1:192959/1‑81 (MQ=255)     gCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATc                                                                       <  1:192921/81‑1 (MQ=255)     gCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATc                                                                       <  2:192922/81‑1 (MQ=255)         cgcCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATCAgg                                                                    <  2:192923/80‑1 (MQ=255)          gcCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATCAGGGc                                                                  <  2:192924/81‑1 (MQ=255)          gcCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATCAGGGc                                                                  >  1:192955/1‑81 (MQ=255)           ccAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATCAGGGcc                                                                 <  2:192925/81‑1 (MQ=255)            cAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATCAGGGc                                                                  >  2:192961/1‑79 (MQ=255)                 gCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATCAGGGCCAGaaaa                                                           >  1:192952/1‑81 (MQ=255)                           tAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATCAGGGCCAGAAAACCCGcagcc                                                  <  2:192926/80‑1 (MQ=255)                             gCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATCAGGGCCAGAAAACCCGcagccagc                                               <  1:192927/81‑1 (MQ=255)                                tGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGt                                             >  1:192953/1‑80 (MQ=255)                                    aaaaCGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCacac                                        >  2:192978/1‑81 (MQ=255)                                      aaCGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCg                                      >  1:192958/1‑81 (MQ=255)                                          tACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCGGTAt                                  >  2:192956/1‑81 (MQ=255)                                            ccGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCGGTATc                                 <  1:192928/80‑1 (MQ=255)                                            ccGAGATCGCGTAATGGCTGGAAAAACTCTGGCACAAGGAGCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCGGTATc                                 <  2:192929/80‑1 (MQ=255)                                               agaTCGCGTAATGGCTGGAAAAACTCTGGCACAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCGGTATCGTAg                             <  1:192930/81‑1 (MQ=255)                                                          tGGCTGGAAAAACTCTGGCACAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCGGTATCGTAGTGACCAAAATc                  <  2:192931/81‑1 (MQ=255)                                                           ggCTGGAAAAACTCTGGCACAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCGGTATCGTAGTGACCAAAATc                  >  2:192967/1‑80 (MQ=255)                                                            gCTGGAAAAACTCTGGCACAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCGGTATCGTAGTGACCAAAATc                  >  2:192964/1‑79 (MQ=255)                                                                gAAAAACTCTGGCACAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCGGTATCGTAGTGACCAAAATCCAGCTc            >  1:192968/1‑81 (MQ=255)                                                                gAAAAACTCTGGCACAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCGGTATCGTAGTGACCAAAATCCAGCTc            <  1:192932/81‑1 (MQ=255)                                                                    aaCTCTGGCACAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCGGTATCGTAGTGACCAAAATCCAGCTCGCCg        <  1:192933/81‑1 (MQ=255)                                                                      ctctGGCACAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCGGTATCGTAGTGACCAAAATCCAGCTCGCCgag      <  1:192934/81‑1 (MQ=255)                                                                          ggCACAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCGGTATTGTAGTGACCAAAATCCAGCTCGCCGAGATAg  >  2:192966/1‑81 (MQ=255)                                                                             |                                                                              GTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCGCAAGGATCAGGGCCAGAAAACCCGCAGCCAGCGTCACACCGGTATCGTAGTGACCAAAATCCAGCTCGCCGAGATAG  >  NC_000913/929997‑930150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑