| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 947,296 | T→C | V23A (GTC→GCC) | ycaM → | putative transporter YcaM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 947,296 | 0 | T | C | 94.4% | 53.9 / ‑6.1 | 18 | V23A (GTC→GCC) | ycaM | putative transporter YcaM |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (8/9); total (9/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTA > NC_000913/947220‑947367 | gATTTGTCTATGGCTGGAAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGTGCTGATGTCTTTTATCACTGCCTgg > 1:196093/1‑81 (MQ=255) tttGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGCCTgggg < 2:196074/81‑1 (MQ=255) tCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGCCTGGGGttt < 2:196075/80‑1 (MQ=255) tGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGCCTGGGGTTTTg < 2:196076/78‑1 (MQ=255) tGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGCCTGGGGTTTTGGc < 2:196077/80‑1 (MQ=255) tGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGCCTGGGGTTTTGGc > 1:196089/1‑80 (MQ=255) ggCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGCCTGGGGTTTTGGCaa > 1:196087/1‑81 (MQ=255) cTGGGAATGTTCAGGAAAAACAGTTTCGATGGTACGACATTACGCTGATGTCTTTTATCGCTGTCTGGGGTTTAGGCAACg > 2:999770/1‑81 (MQ=255) gAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGCCTGGGGTTTTGGCAACgttgt < 1:196078/81‑1 (MQ=255) tGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGCCTGGGGTTTTGGCAACGTTGTtaa < 1:196079/81‑1 (MQ=255) aaaCAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGCCTGGGGTTTTGGCAACGTTGTTAATAACTATGcc < 2:196080/81‑1 (MQ=255) aaaCAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGCCTGGGGTTTTGGCAACGTTGTTAATAACTATGc > 1:196094/1‑80 (MQ=255) cAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGCCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAAc > 2:196096/1‑81 (MQ=255) gCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGCCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAgg < 1:196081/80‑1 (MQ=255) gcTGATGTCTTTTATCACTGCCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGtttttt > 1:196095/1‑80 (MQ=255) gTCTTTTATCACTGCCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGgt > 1:196102/1‑81 (MQ=255) ttATCACTGCCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGtt < 1:196082/79‑1 (MQ=255) ttATCACTGCCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTa > 2:196097/1‑81 (MQ=255) | GATTTGTCTATGGCTGGGAATGTTCAGGAAAAACAGTTGCGATGGTACAACATTGCGCTGATGTCTTTTATCACTGTCTGGGGTTTTGGCAACGTTGTTAATAACTATGCCAACCAGGGGCTGGTGGTTGTTTTTTCATGGGTGTTTA > NC_000913/947220‑947367 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |