Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	978,267	G→A	E648K (GAA→AAA)	mukB →	chromosome partitioning protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	978,267	0	G	A	100.0%	94.8 / NA	27	E648K (GAA→AAA)	mukB	chromosome partitioning protein MukB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/16);  total (11/16)

CCTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCG  >  NC_000913/978193‑978343                                                                           |                                                                             ccTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTgg                                                                        <  1:201515/81‑1 (MQ=255)     cagccagGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGgcg                                                                     <  2:201516/80‑1 (MQ=255)      agccagGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGgcgcg                                                                   <  1:201517/81‑1 (MQ=255)             aCGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCTATTGTTGAACGCGATAAAGTGGGCGCGCGCaaa                                                             >  1:201572/1‑80 (MQ=255)               gTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAAc                                                          <  2:201518/81‑1 (MQ=255)                 cACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGc                                                        >  1:201554/1‑81 (MQ=255)                   ccGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCg                                                      <  2:201519/81‑1 (MQ=255)                    cGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGt                                                     <  2:201520/81‑1 (MQ=255)                     gAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTc                                                    >  1:201565/1‑81 (MQ=255)                     gAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTc                                                    <  1:201521/81‑1 (MQ=255)                          tCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATg                                                <  2:201523/80‑1 (MQ=255)                          tCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATg                                                <  2:201522/80‑1 (MQ=255)                              cAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAag                                             <  1:201524/79‑1 (MQ=255)                                aCAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGAt                                         <  2:201526/81‑1 (MQ=255)                                aCAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGAt                                         <  2:201525/81‑1 (MQ=255)                                 cAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGaa                                              >  2:201564/1‑75 (MQ=255)                                  aGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATc                                        >  1:201546/1‑80 (MQ=255)                                      gCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCTGTCGATGAAGAGATCGAAc                                    <  1:201527/80‑1 (MQ=255)                                          gAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGtt                                 <  1:201528/79‑1 (MQ=255)                                           aGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTaa                              <  1:201529/81‑1 (MQ=255)                                                      gagGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTggc                   >  2:201567/1‑81 (MQ=255)                                                      gagGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTggc                   >  1:201573/1‑81 (MQ=255)                                                        ggCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTggcgg                 <  1:201530/81‑1 (MQ=255)                                                         gCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTggcggc                >  1:201570/1‑81 (MQ=255)                                                                   aaCGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGAt       >  1:201563/1‑80 (MQ=255)                                                                    aCGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGAt       >  1:201574/1‑79 (MQ=255)                                                                       cgATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCg  >  1:201558/1‑81 (MQ=255)                                                                           |                                                                             CCTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCG  >  NC_000913/978193‑978343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑