Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	981,907	C→A	S287S (TCC→TCA)	ldtD →	L,D‑transpeptidase LdtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	981,907	0	C	A	100.0%	114.1 / NA	32	S287S (TCC→TCA)	ldtD	L,D‑transpeptidase LdtD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/14);  total (18/14)

CACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGCCGT  >  NC_000913/981829‑981984                                                                               |                                                                              cacaGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGc                                                                             <  1:202199/81‑1 (MQ=255)    aGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGt                                                                          >  2:202231/1‑81 (MQ=255)     ggCATTTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGt                                                                          >  2:202240/1‑80 (MQ=255)      gCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTa                                                                        <  1:202200/81‑1 (MQ=255)        aTGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACt                                                                      <  2:202201/81‑1 (MQ=255)        aTGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACt                                                                      >  1:202215/1‑81 (MQ=255)           ttGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGtt                                                                   <  1:202202/81‑1 (MQ=255)              gACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGa                                                                 >  2:202234/1‑80 (MQ=255)              gACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGa                                                                 >  1:202243/1‑80 (MQ=255)                 ggggggCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAAca                                                             <  1:202203/81‑1 (MQ=255)                      gCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAAcagcag                                                         >  2:202248/1‑80 (MQ=255)                           aaaTTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTa                                                   <  1:202204/81‑1 (MQ=255)                             aTTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAg                                                 <  2:202205/81‑1 (MQ=255)                                aCTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGct                                               <  2:202206/80‑2 (MQ=255)                                aCTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGcc                                               <  1:202207/80‑1 (MQ=255)                                    tACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATg                                           >  1:202245/1‑80 (MQ=255)                                         ggCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAg                                     >  2:202259/1‑81 (MQ=255)                                         ggCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAg                                     >  1:202275/1‑81 (MQ=255)                                         ggCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAg                                     >  2:202255/1‑81 (MQ=255)                                           cGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGc                                    >  1:202263/1‑80 (MQ=255)                                              tGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAac                                >  2:202238/1‑81 (MQ=255)                                                  acGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAacgacg                            >  2:202253/1‑81 (MQ=255)                                                  acGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAacgacg                            <  2:202208/81‑1 (MQ=255)                                                       aaCTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCg                       >  1:202250/1‑81 (MQ=255)                                                             cgcgGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTaa                 <  1:202209/81‑1 (MQ=255)                                                               cgGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAAc               <  2:202210/81‑1 (MQ=255)                                                                  tAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTg            >  2:202254/1‑81 (MQ=255)                                                                   aGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTgc           >  2:202246/1‑81 (MQ=255)                                                                       aGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCt       >  2:202249/1‑81 (MQ=255)                                                                       aGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCt       <  1:202211/81‑1 (MQ=255)                                                                            aTCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGCCGt  >  1:202257/1‑81 (MQ=255)                                                                            aTCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGCCGt  <  1:202212/81‑1 (MQ=255)                                                                               |                                                                              CACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGCCGT  >  NC_000913/981829‑981984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑