Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	984,036	C→T	C129C (TGC→TGT)	gloC →	hydroxyacylglutathione hydrolase GloC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	984,036	0	C	T	100.0%	103.7 / NA	29	C129C (TGC→TGT)	gloC	hydroxyacylglutathione hydrolase GloC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/17);  total (12/17)

ACGCCAGATCGTTGGCTGAACGAAGGCGATACCATCAGCATAGGGAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAA  >  NC_000913/983965‑984113                                                                        |                                                                              aCGCCAGATCGTTGGCTGAACGAAGGCGATACCATCAGCATAGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCAt                                                                      <  1:202578/81‑1 (MQ=255)    ccAGATCGTTGGCTGAACGAAGGCGATACCATCAGCATAGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACg                                                                   >  1:202624/1‑81 (MQ=255)        aTCGTTGGCTGAACGAAGGCGATACCATCAGCATAGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCgg                                                               <  1:202579/81‑1 (MQ=255)           gTTGGCTGAACGAAGGCGATACCATCAGCATGGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTc                                                            <  2:202580/81‑1 (MQ=255)              ggCTGAACGAAGGCGATACCATCAGCATAGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCAt                                                          <  2:202581/80‑1 (MQ=255)               gCTGAACGAAGGCGATACCATCAGCATAGGGGATGTGACTTTGCAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGt                                                        <  2:202582/81‑1 (MQ=255)                cTGAACGAAGGCGATACCATCAGCATAGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTc                                                       <  2:202583/81‑1 (MQ=255)                  gAACGAAGGCGATACCATCAGCATAGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCgt                                                     >  2:202627/1‑81 (MQ=255)                      gAAGGCGATACCATCAGCATAGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGttt                                                 >  1:202622/1‑81 (MQ=255)                                ccATCAGCATAGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTgatgat                                        <  1:202584/80‑1 (MQ=255)                                    cAGCATAGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCggg                                    >  1:202644/1‑80 (MQ=255)                                     aGCATAGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGc                                   >  1:202621/1‑80 (MQ=255)                                      gCATAGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCaa                                 <  1:202585/81‑1 (MQ=255)                                       cATAGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCa                                  >  1:202623/1‑79 (MQ=255)                                        aTAGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAg                               <  1:202586/81‑1 (MQ=255)                                        aTAGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAg                               >  1:202625/1‑81 (MQ=255)                                          aGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAgct                             >  2:202640/1‑81 (MQ=255)                                          aGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAgct                             >  1:202633/1‑81 (MQ=255)                                          aGGGAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGCTTTTTGATGATCGGGCAAAgct                             >  1:202613/1‑81 (MQ=255)                                           gggAATGTGACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAgctg                            <  1:202587/81‑1 (MQ=255)                                             gagtgtgACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAgctgct                          <  2:202588/78‑1 (MQ=255)                                                  tgACTTTACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGAttt                     <  1:202589/81‑1 (MQ=255)                                                       ttACAGGTGTTACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGc                >  1:202630/1‑81 (MQ=255)                                                                ttACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTAtt       <  2:202590/81‑1 (MQ=255)                                                                ttACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTAt        <  2:202591/80‑1 (MQ=255)                                                                 tACATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTAttt      >  1:202635/1‑81 (MQ=255)                                                                   cATTGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTAtttt     <  1:202592/80‑1 (MQ=255)                                                                     ttGTCCTGTGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGTGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCaa  <  1:202593/81‑1 (MQ=255)                                                                     ttGTCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCaa  <  1:202594/81‑1 (MQ=255)                                                                        |                                                                              ACGCCAGATCGTTGGCTGAACGAAGGCGATACCATCAGCATAGGGAATGTGACTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAA  >  NC_000913/983965‑984113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑