Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	80,792	A→G	L25L (TTA→CTA)	leuC ←	3‑isopropylmalate dehydratase subunit LeuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	80,792	0	A	G	100.0%	86.7 / NA	26	L25L (TTA→CTA)	leuC	3‑isopropylmalate dehydratase subunit LeuC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/12);  total (14/12)

CGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCA  >  NC_000913/80723‑80863                                                                      |                                                                        cGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAGCAGTGGGGttt                                                              <  2:19704/81‑1 (MQ=255)   tGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAGCAGTGGGGTTTCg                                                            >  2:19753/1‑81 (MQ=255)        gcgcAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTc                                                       >  2:19763/1‑81 (MQ=255)               ccATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCg                                                <  1:19705/81‑1 (MQ=255)                cATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCg                                                >  1:19761/1‑80 (MQ=255)                   cGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTaca                                            >  1:19746/1‑81 (MQ=255)                             cGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGc                                  <  1:19706/81‑1 (MQ=255)                                  aGGTCACTTCATGCACCAGGTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCg                              >  1:19737/1‑80 (MQ=255)                                  aGGTCACTTCATGCACCAGGTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGa                             <  2:19707/81‑1 (MQ=255)                                    gTCACTTCATGCACCAGGTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAAc                           <  2:19708/81‑1 (MQ=255)                                        cTTCATGCACCAGGTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAAtt                       >  1:19777/1‑81 (MQ=255)                                         ttCATGCACCAGGTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAAttt                      >  2:19755/1‑81 (MQ=255)                                            aTGCACCAGGTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTc                   <  2:19709/81‑1 (MQ=255)                                                 ccAGGTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGtat               >  1:19757/1‑80 (MQ=255)                                                 ccAGGTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGtat               >  2:19760/1‑80 (MQ=255)                                                  cAGGTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGtat               <  2:19710/79‑1 (MQ=255)                                                     gTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGt          >  1:19747/1‑81 (MQ=255)                                                     gTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGt          <  2:19711/81‑1 (MQ=255)                                                     gTGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGt          >  2:19756/1‑81 (MQ=255)                                                      tGGCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTc         <  2:19712/81‑1 (MQ=255)                                                        gCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCtt       <  1:19713/81‑1 (MQ=255)                                                        gCGGTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCt        >  2:19788/1‑80 (MQ=255)                                                          ggTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAg     >  1:19749/1‑81 (MQ=255)                                                          ggTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAg     >  2:19751/1‑81 (MQ=255)                                                          ggTCGATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAg     <  1:19714/81‑1 (MQ=255)                                                              gATATATAGCAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCa  <  2:19715/80‑1 (MQ=255)                                                                      |                                                                        CGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCA  >  NC_000913/80723‑80863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑