Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	989,174	G→A	H395Y (CAT→TAT)	pncB ←	nicotinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	989,174	0	G	A	100.0%	68.5 / NA	20	H395Y (CAT→TAT)	pncB	nicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/8);  total (12/8)

AAAGATTTCGGAATTAAAGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATGCGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAG  >  NC_000913/989100‑989248                                                                           |                                                                           aaaGATTTCGGAATTAAAGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAg                                                                      >  2:203481/1‑81 (MQ=255)   aGATTTCGGAATTAAAGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGt                                                                    >  2:203478/1‑81 (MQ=255)         cGGAATTAAAGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACg                                                              >  2:203482/1‑81 (MQ=255)            aaTTAAAGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCtt                                                           >  1:203491/1‑81 (MQ=255)                 aaGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTgcgc                                                      <  2:203456/81‑1 (MQ=255)                    gTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAgc                                                   >  1:203484/1‑81 (MQ=255)                            aGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAAc                                           <  2:203457/81‑1 (MQ=255)                             gggAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACa                                          <  1:203458/81‑1 (MQ=255)                              ggAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACa                                          >  1:203485/1‑80 (MQ=255)                                aGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAAc                                       >  1:203479/1‑81 (MQ=255)                                        aGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTAt                                >  1:203493/1‑80 (MQ=255)                                            cccTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATgg                           >  2:203486/1‑81 (MQ=255)                                                 atgatATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAg                        <  1:203459/79‑1 (MQ=255)                                                       ttAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGttt                 <  1:203460/80‑1 (MQ=255)                                                          aCTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGc              >  1:203488/1‑80 (MQ=255)                                                          aCTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGc              >  2:203483/1‑80 (MQ=255)                                                              gCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAggg         <  1:203461/81‑1 (MQ=255)                                                                  ttttAATATACGGAAGGTCGAACGCTCTGTGCAGCGCCCGAACATACGCTTTATCATGGCAGATAGTTTTGCCAGGGTTGt     >  1:203489/1‑81 (MQ=255)                                                                      aaTATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAg  <  2:203462/80‑1 (MQ=255)                                                                      aaTATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAg  <  1:203463/80‑1 (MQ=255)                                                                           |                                                                           AAAGATTTCGGAATTAAAGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATGCGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAG  >  NC_000913/989100‑989248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑