Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	995,400	A→G	W197R (TGG→CGG)	ssuD ←	FMNH2‑dependent alkanesulfonate monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	995,400	0	A	G	100.0%	97.6 / NA	29	W197R (TGG→CGG)	ssuD	FMNH2‑dependent alkanesulfonate monooxygenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/14);  total (15/14)

TTTTGCGTCCATGCGCGGCAGCTTTCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCAGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCAAAGTAAAGTG  >  NC_000913/995325‑995471                                                                            |                                                                        ttttGCGTCCATGCGCGGCAGCTTTCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGt                                                                      >  1:204659/1‑79 (MQ=255)  tttGCGTCCATGCGCGGCAGCTTTCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAg                                                                   >  1:204687/1‑81 (MQ=255)   ttGCGTCCATGCGCGGCAGCTTTCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAg                                                                   <  2:204632/80‑1 (MQ=255)         ccATGCGCGGCAGCTTTCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTa                                                                >  1:204680/1‑77 (MQ=255)                 ggCAGCTTTCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTg                                                    >  1:204679/1‑81 (MQ=255)                       tttCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTgc                                              >  1:204678/1‑81 (MQ=255)                         tCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTgcc                                             <  1:204633/80‑1 (MQ=255)                           gCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTgccgc                                           >  2:204690/1‑80 (MQ=255)                           gCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTgccgc                                           >  2:204684/1‑80 (MQ=255)                             ccGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAg                                        >  2:204689/1‑81 (MQ=255)                              cGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGc                                       <  2:204634/81‑1 (MQ=255)                                 aCTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTcc                                    <  2:204635/81‑1 (MQ=255)                                        cGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTTGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGa                             >  2:204693/1‑81 (MQ=255)                                         gATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGAc                            <  1:204637/81‑1 (MQ=255)                                         gATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGAc                            <  2:204636/81‑1 (MQ=255)                                          aTTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCCACCTGTTCTGCCGCCAGCTCCTGGGCGACa                           <  1:204638/81‑1 (MQ=255)                                              tctcTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCt                        <  2:204639/80‑1 (MQ=255)                                              tctcTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCt                        >  2:204676/1‑80 (MQ=255)                                                    tAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACg                    <  2:204640/78‑1 (MQ=255)                                                     aaCCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATc                 <  2:204641/80‑1 (MQ=255)                                                        cAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGcc             <  1:204642/81‑1 (MQ=255)                                                          gTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCaa           <  2:204644/81‑1 (MQ=255)                                                          gTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCaa           <  1:204643/81‑1 (MQ=255)                                                            tCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCaaag         >  2:204695/1‑81 (MQ=255)                                                              cggcggTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCaaagt        >  1:204682/1‑80 (MQ=255)                                                              cggcggTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCaaagt        >  2:204691/1‑80 (MQ=255)                                                               ggcggTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCaaagta       >  2:204683/1‑80 (MQ=255)                                                               ggcggTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCaaagta       >  1:204666/1‑80 (MQ=255)                                                                   gTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCAAAGTAAAGTg  <  2:204645/81‑1 (MQ=255)                                                                            |                                                                        TTTTGCGTCCATGCGCGGCAGCTTTCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCAGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCAAAGTAAAGTG  >  NC_000913/995325‑995471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑