Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,019,171	T→A	I295F (ATC→TTC)	ompA ←	outer membrane porin A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,019,171	0	T	A	100.0%	68.5 / NA	20	I295F (ATC→TTC)	ompA	outer membrane porin A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/9);  total (11/9)

AGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCT  >  NC_000913/1019095‑1019242                                                                             |                                                                        aGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTg                                                                     >  2:208543/1‑81 (MQ=255)               tttCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATAc                                                        <  1:208505/80‑1 (MQ=255)                ttCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCt                                                      >  2:624103/1‑81 (MQ=255)                     gTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTg                                                   >  2:208546/1‑79 (MQ=255)                      ttGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTg                                                   >  1:208535/1‑78 (MQ=255)                      ttGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGgag                                                <  2:208506/81‑1 (MQ=255)                         tCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATc                                             <  1:208507/81‑1 (MQ=255)                         tCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATc                                             <  1:208508/81‑1 (MQ=255)                                    gCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATcaa                                   >  2:208544/1‑80 (MQ=255)                                       aGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATcaacaa                                >  1:208533/1‑80 (MQ=255)                                          aaCCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGa                            >  2:208536/1‑81 (MQ=255)                                           aCCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGAc                           >  2:208542/1‑81 (MQ=255)                                                    gATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACgg                  <  2:208509/81‑1 (MQ=255)                                                      ttCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGgcg                >  1:208550/1‑81 (MQ=255)                                                         gCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTc             <  2:208510/81‑1 (MQ=255)                                                           ccATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCgg           <  1:455907/81‑1 (MQ=255)                                                                ccACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAg       <  2:208511/80‑1 (MQ=255)                                                                  aCGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGAcc    >  1:208538/1‑81 (MQ=255)                                                                    gTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCt  <  2:208512/81‑1 (MQ=255)                                                                    gTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCt  >  1:208532/1‑81 (MQ=255)                                                                             |                                                                        AGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCT  >  NC_000913/1019095‑1019242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑