Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,022,536	C→T	A450T (GCC→ACC)	yccS ←	putative transporter YccS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,022,536	0	C	T	100.0%	101.3 / NA	29	A450T (GCC→ACC)	yccS	putative transporter YccS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/14);  total (15/14)

CGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGA  >  NC_000913/1022464‑1022607                                                                         |                                                                        cGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGAtaccta                                                                 <  2:209070/81‑1 (MQ=255)  gCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTTAtacctac                                                                >  1:209107/1‑81 (MQ=255)     ggTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACTGCACAGGAATGCCAATGGTGATACCTACCAg                                                             >  2:209105/1‑81 (MQ=255)      gTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGc                                                            <  2:209072/81‑1 (MQ=255)      gTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGc                                                            <  2:209071/81‑1 (MQ=255)            aCCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTAcc                                                       <  1:209073/80‑1 (MQ=255)            aCCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTAcc                                                       >  2:209104/1‑80 (MQ=255)              cagcagCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCaat                                                    <  2:209074/81‑1 (MQ=255)              cagcagCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCaa                                                     >  1:209111/1‑80 (MQ=255)                gcagcaCCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCaataa                                                  >  2:209123/1‑81 (MQ=255)                  agcaCCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCAATAATc                                                <  2:209075/81‑1 (MQ=255)                   gcaCCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCAATAATcc                                               >  2:209110/1‑81 (MQ=255)                       cAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTaa                                           >  2:209100/1‑81 (MQ=255)                           tGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACtt                                        >  1:209108/1‑80 (MQ=255)                             cccTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAGCTTc                                       <  2:209076/79‑1 (MQ=255)                             cccTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACTTCAg                                     >  1:209112/1‑81 (MQ=255)                                     gTGATGGCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGgc                             <  2:209077/81‑1 (MQ=255)                                          tgCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGgcgcact                        >  2:95731/2‑78 (MQ=255)                                          ggCACAAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTg                        >  2:209116/1‑81 (MQ=255)                                              caAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGt                    <  2:209078/81‑1 (MQ=255)                                              caAACCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGt                    >  1:209114/1‑81 (MQ=255)                                                aaCCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTta                  <  2:209079/81‑1 (MQ=255)                                                aaCCACAGCACAGGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTta                  >  2:209117/1‑81 (MQ=255)                                                    acagcacagGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGt              <  1:209080/81‑1 (MQ=255)                                                     cagcacagGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGtt             <  2:209081/81‑1 (MQ=255)                                                        cacagGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTtgg          >  1:209109/1‑81 (MQ=255)                                                        cacagGAATGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTtgg          <  1:209082/81‑1 (MQ=255)                                                               aTGCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAg   <  2:209083/81‑1 (MQ=255)                                                                 gCCAATGGTGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGa  >  1:209113/1‑80 (MQ=255)                                                                         |                                                                        CGCCGGTAATAACCAGCAGCACCAGCTGCCCTTCCAGTGATGGCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGA  >  NC_000913/1022464‑1022607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑