Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,034,437	A→G	Y395C (TAC→TGC)	hyaB →	hydrogenase 1 large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,034,437	0	A	G	100.0%	106.5 / NA	31	Y395C (TAC→TGC)	hyaB	hydrogenase 1 large subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/14);  total (17/14)

CACCGACCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTACTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTAT  >  NC_000913/1034360‑1034513                                                                              |                                                                             cACCGACCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTc                                                                           >  1:211243/1‑81 (MQ=255) cACCGACCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTc                                                                           >  1:211244/1‑81 (MQ=255) cACCGACCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTc                                                                           >  1:211258/1‑81 (MQ=255)      aCCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGa                                                                      >  1:211247/1‑81 (MQ=255)          gTGGTACAACCCCGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCa                                                                   >  1:211256/1‑80 (MQ=255)           tGGTACAACCCCGGCGATGTCAAATGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCa                                                                   >  1:211254/1‑79 (MQ=255)             gTACAACCCCGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAgc                                                               <  2:211207/81‑1 (MQ=255)                  aCCCCGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCAc                                                          >  2:211252/1‑81 (MQ=255)                   ccccGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACg                                                         >  1:211263/1‑81 (MQ=255)                      cGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTg                                                      >  2:211249/1‑81 (MQ=255)                         cGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGgcg                                                   <  2:211208/81‑1 (MQ=255)                          gATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGgcgc                                                  >  1:211259/1‑81 (MQ=255)                           aTGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGcgcg                                                 <  1:211209/81‑1 (MQ=255)                              tCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGt                                               <  2:211210/80‑1 (MQ=255)                                aaaGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAAc                                            <  1:211211/81‑1 (MQ=255)                                aaaGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAAc                                            <  1:211212/81‑1 (MQ=255)                                     cAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGAt                                       <  1:211213/81‑1 (MQ=255)                                      aGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATg                                      >  1:211269/1‑81 (MQ=255)                                      aGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATg                                      <  2:211214/81‑1 (MQ=255)                                          aTACCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAg                                  >  1:211283/1‑81 (MQ=255)                                            aCCAACATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTg                                >  1:211250/1‑81 (MQ=255)                                               aaCATTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTgggg                             >  1:211261/1‑81 (MQ=255)                                                  aTTCAGCAGCTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCg                          <  2:211216/81‑1 (MQ=255)                                                      agcagcTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTgg                      <  1:211217/81‑1 (MQ=255)                                                      agcagcTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTgg                      >  2:211273/1‑81 (MQ=255)                                                        cagcTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGgcg                    >  1:211262/1‑81 (MQ=255)                                                           cTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGgcgcgc                 <  2:211218/81‑1 (MQ=255)                                                           cTGAATGAACAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGgcgcgc                 <  2:211219/81‑1 (MQ=255)                                                            tgaatgaaCAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCa                >  1:211267/1‑81 (MQ=255)                                                              aatgaaCAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCa                <  2:211220/79‑1 (MQ=255)                                                                    cAGGAACGCTGCTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATc        <  1:211221/81‑1 (MQ=255)                                                                           gctgctCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTAt  >  2:211276/1‑80 (MQ=255)                                                                              |                                                                             CACCGACCCGTGGTACAACCCCGGCGATGTCAAAGGCAGCGATACCAACATTCAGCAGCTGAATGAACAGGAACGCTACTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTAT  >  NC_000913/1034360‑1034513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑