Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,064,853	A→T	D273V (GAT→GTT)	yccE →	uncharacterized protein YccE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,064,853	0	A	T	100.0%	23.5 / NA	8	D273V (GAT→GTT)	yccE	uncharacterized protein YccE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (2/6);  total (2/6)

GAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTAAAAAA  >  NC_000913/1064789‑1064923                                                                 |                                                                       gAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAAc                                                        >  1:216539/1‑81 (MQ=255)  aGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAAc                                                        >  1:216536/1‑80 (MQ=255)           ttgATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAgg                                              <  1:216518/81‑1 (MQ=255)                    cATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGagta                                     <  1:216519/81‑1 (MQ=255)                             aTCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGt                            <  2:216520/81‑1 (MQ=255)                                  aGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCAt                         <  2:216521/79‑1 (MQ=255)                                         aTGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTc                <  1:216522/81‑1 (MQ=255)                                                       tttGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTaaaaaa  <  1:216523/81‑1 (MQ=255)                                                                 |                                                                       GAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTAAAAAA  >  NC_000913/1064789‑1064923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑