| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,065,263 | C→T | L410F (CTC→TTC) | yccE → | uncharacterized protein YccE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,065,263 | 0 | C | T | 92.9% | 39.2 / ‑3.7 | 14 | L410F (CTC→TTC) | yccE | uncharacterized protein YccE |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (9/4); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTT > NC_000913/1065191‑1065340 | tATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAATTCGACTAt > 2:216562/1‑81 (MQ=255) aTGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTAtt > 1:216563/1‑81 (MQ=255) cGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAATTCGACTATTTaaa > 1:216567/1‑81 (MQ=255) gTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAATTCGACTATTTAAAt > 1:216561/1‑81 (MQ=255) gTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAATTCGACTATTTAAAt > 1:216564/1‑81 (MQ=255) cAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAATTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCa > 2:216565/1‑80 (MQ=255) cAAGTTAAACAATAAGGAAATGAATGCGCAATTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTaaa < 2:216550/81‑1 (MQ=255) aaaCAATAAGGAAATGAATGCGCAATTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCAt > 1:216570/1‑81 (MQ=255) aaaCAATAAGGAAATGAATGCGCAATTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCAt > 1:216579/1‑81 (MQ=255) aaCAATAAGGAAATGAATGCGCAATTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCAtt < 1:216551/81‑1 (MQ=255) aatgaatgCGCAATTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGtct < 2:216552/81‑1 (MQ=255) aatgCGCAATTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTcaca > 2:216577/1‑81 (MQ=255) atgCGCAATTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCacac > 2:216571/1‑81 (MQ=255) cAATTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACttttt < 2:216553/81‑1 (MQ=255) | TATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTT > NC_000913/1065191‑1065340 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |