Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,068,977	C→G	G288A (GGC→GCC)	rutG ←	pyrimidine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,068,977	0	C	G	100.0%	98.1 / NA	29	G288A (GGC→GCC)	rutG	pyrimidine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (13/16);  total (13/16)

ACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACC  >  NC_000913/1068904‑1069047                                                                          |                                                                       acCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGaa                                                                 <  1:217061/81‑1 (MQ=255) acCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGaa                                                                 >  2:217112/1‑81 (MQ=255) acCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGaa                                                                 >  2:217109/1‑81 (MQ=255)  ccGATATTTTCAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAAt                                                                <  1:217062/81‑1 (MQ=255)                aTAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCAtata                                                  >  2:217117/1‑81 (MQ=255)                aTAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCAtata                                                  >  2:217102/1‑81 (MQ=255)                 tAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATAc                                                 >  1:217100/1‑81 (MQ=255)                  aGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACg                                                >  2:217105/1‑81 (MQ=255)                   ggtggtCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACgg                                               <  2:217064/81‑1 (MQ=255)                   ggtggtCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACgg                                               <  2:217063/81‑1 (MQ=255)                     tggtCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGt                                             >  1:217101/1‑81 (MQ=255)                           cgccgcTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCatat                                       <  1:217065/81‑1 (MQ=255)                            gccgcTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATAtt                                      <  2:217066/81‑1 (MQ=255)                              cgcTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGc                                    >  2:217110/1‑81 (MQ=255)                                   ccgccgACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCg                               <  2:217068/81‑1 (MQ=255)                                   ccgccgACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGAGTCCg                               <  1:217067/81‑1 (MQ=255)                                     gccgACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGt                             >  2:217099/1‑81 (MQ=255)                                      ccgACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTc                            <  1:217070/81‑1 (MQ=255)                                      ccgACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTc                            <  1:217069/81‑1 (MQ=255)                                           agagCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATccc                       >  1:217107/1‑81 (MQ=255)                                           agagCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATccc                       <  2:217071/81‑1 (MQ=255)                                              gCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGc                    <  1:217072/81‑1 (MQ=255)                                               ccGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCg                   <  1:217073/81‑1 (MQ=255)                                                 ggAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGAc                 >  1:217113/1‑81 (MQ=255)                                                        aTCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTg          <  2:217074/81‑1 (MQ=255)                                                           gTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCtt           >  1:217115/1‑77 (MQ=255)                                                              gCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATg     <  1:217075/80‑1 (MQ=255)                                                                cAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGAcc  <  2:217076/81‑1 (MQ=255)                                                                 aGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGAcc  >  1:217124/1‑80 (MQ=255)                                                                          |                                                                       ACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACC  >  NC_000913/1068904‑1069047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑