Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	89,913	A→G	I94V (ATC→GTC)	mraZ →	DNA‑binding transcriptional repressor MraZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	89,913	0	A	G	100.0%	115.9 / NA	34	I94V (ATC→GTC)	mraZ	DNA‑binding transcriptional repressor MraZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/19);  total (15/19)

CCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAATCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTGGTTGGACAGTTCAACAAGTTTGAGCT  >  NC_000913/89835‑89989                                                                               |                                                                             ccGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTc                                                                            <  2:21642/81‑1 (MQ=255)         gcgcCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGt                                                                    <  1:21643/81‑1 (MQ=255)           gcCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTAc                                                                  >  1:21679/1‑81 (MQ=255)            ccGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACt                                                                 <  2:21644/81‑1 (MQ=255)             cGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTg                                                                <  2:21645/81‑1 (MQ=255)              gtgtGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGc                                                               <  1:21646/81‑1 (MQ=255)              gtgtGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGc                                                               <  1:21647/81‑1 (MQ=255)                 tgCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCgg                                                             >  1:21692/1‑80 (MQ=255)                  gCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGc                                                            >  2:21699/1‑80 (MQ=255)                    aGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCaa                                                          >  2:21704/1‑80 (MQ=255)                       gcCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATg                                                      <  2:21648/81‑1 (MQ=255)                         cTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGcc                                                    <  2:21649/81‑1 (MQ=255)                         cTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGcc                                                    <  2:21650/81‑1 (MQ=255)                                  ggTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGAc                                            <  2:21651/80‑1 (MQ=255)                                     cATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACaa                                          >  1:21681/1‑79 (MQ=255)                                        gCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGTCAGTACTGCGGCAACATGCCGGGCTGACAAaag                                       >  1:21691/1‑79 (MQ=255)                                         ccAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAaagaa                                     <  2:21652/80‑1 (MQ=255)                                           aGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTg                                  <  2:21653/81‑1 (MQ=255)                                              gAATGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATg                               >  2:21700/1‑81 (MQ=255)                                               aaTGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGc                              <  1:21654/81‑1 (MQ=255)                                                aTGTCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCt                             >  2:21709/1‑81 (MQ=255)                                                   tCAGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTgg                           <  1:21655/80‑1 (MQ=255)                                                     aGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTGGtt                         <  2:21656/80‑1 (MQ=255)                                                     aGATGGATGGCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTGGtt                         <  2:21657/80‑1 (MQ=255)                                                        tggatggCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTGGTTGGa                      >  2:21701/1‑80 (MQ=255)                                                        tggatggCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTGGTTGGAc                     <  1:21658/81‑1 (MQ=255)                                                         ggatggCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTGGTTGGa                      >  1:21694/1‑79 (MQ=255)                                                         ggatggCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTGGTTGGa                      >  2:21697/1‑79 (MQ=255)                                                            tggCGCAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTGGTTGGACAGtt                 >  1:21743/1‑81 (MQ=255)                                                               cgcAGGTCGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTGGTTGGACAGTTcaa              >  1:21695/1‑81 (MQ=255)                                                                      cGATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTGGTTGGACAGTTCAACAAGttt       >  2:21707/1‑81 (MQ=255)                                                                       gATTGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTGGTTGGACAGTTCAACAAGttt       >  1:21713/1‑80 (MQ=255)                                                                         ttGTTAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTGGTTGGACAGTTCAACAAGTTTGAg    <  2:21659/81‑1 (MQ=255)                                                                            ttAGTCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTGGTTGGACAGTTCAACAAGTTTGAGCt  <  2:21660/80‑1 (MQ=255)                                                                               |                                                                             CCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAATCGCGCCAGTACTGCGGCAACATGCCGGGCTGACAAAAGAAGTGATGCTGGTTGGACAGTTCAACAAGTTTGAGCT  >  NC_000913/89835‑89989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑