Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,096,234	C→G	P131R (CCG→CGG)	ycdU →	uncharacterized protein YcdU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,096,234	0	C	G	100.0%	62.7 / NA	19	P131R (CCG→CGG)	ycdU	uncharacterized protein YcdU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (10/9);  total (10/9)

CAATGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCCGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCG  >  NC_000913/1096165‑1096304                                                                      |                                                                       cAATGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCt                                                             <  2:221459/81‑1 (MQ=255)  aaTGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCtt                                                            <  1:221460/81‑1 (MQ=255)   aTGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCttt                                                           >  1:221465/1‑81 (MQ=255)          gggggCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGt                                                    >  2:221475/1‑81 (MQ=255)           ggggCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGtt                                                   >  2:221474/1‑81 (MQ=255)            gggCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGttt                                                  >  1:221481/1‑81 (MQ=255)                  taCCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGgatg                                            <  1:221461/81‑1 (MQ=255)                            tACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCAc                                    >  2:221483/1‑79 (MQ=255)                             aCCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAg                                 >  1:221479/1‑81 (MQ=255)                              cccTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGt                                <  1:221462/81‑1 (MQ=255)                                 tCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTAt                             >  1:221484/1‑81 (MQ=255)                                        atGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAg                       >  1:221482/1‑80 (MQ=255)                                         tGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGc                      <  2:221463/80‑1 (MQ=255)                                          gcgcTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAg                    >  1:221488/1‑81 (MQ=255)                                           cgcTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAgg                   <  2:221464/81‑1 (MQ=255)                                                     gATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTAttt          >  1:221485/1‑80 (MQ=255)                                                          cGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTa     <  2:221465/80‑1 (MQ=255)                                                           gTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGc   <  1:221466/81‑1 (MQ=255)                                                            ttACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCg  <  2:221467/81‑1 (MQ=255)                                                                      |                                                                       CAATGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCCGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCG  >  NC_000913/1096165‑1096304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑