Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,100,298	G→A	M1M (ATG→ATA) †	ycdZ →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,100,298	0	G	A	100.0%	77.5 / NA	22	M1M (ATG→ATA) †	ycdZ	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/7);  total (15/7)

AACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTT  >  NC_000913/1100221‑1100375                                                                              |                                                                              aaCGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATaaa                                                                             >  1:222071/1‑80 (MQ=255)     gATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATAc                                                                        >  2:222078/1‑81 (MQ=255)       tAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACtt                                                                      >  1:222074/1‑81 (MQ=255)        aaTTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTtc                                                                     >  2:222063/1‑81 (MQ=255)            tgttgttgCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTtctct                                                                  <  1:222042/80‑1 (MQ=255)             gttgttgCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTtctctc                                                                 <  2:222043/80‑1 (MQ=255)                 ttgCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTAtt                                                             <  2:222044/80‑1 (MQ=255)                         aaTGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATcac                                                     >  1:222072/1‑80 (MQ=255)                                gCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAATATACTTCTCTCTATTGCAATCACAACAGGCa                                             >  2:222080/1‑81 (MQ=255)                                     aTCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATtctc                                        >  1:222075/1‑81 (MQ=255)                                        tcattcatGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTcc                                     >  1:222067/1‑81 (MQ=255)                                          attcatGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATtctct                                       <  1:222045/77‑1 (MQ=255)                                           ttcatGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCgg                                   >  1:222077/1‑80 (MQ=255)                                                 gCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTgg                            >  2:222069/1‑81 (MQ=255)                                                    gcgTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGa                         >  1:222087/1‑81 (MQ=255)                                                          tCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTg                   >  1:222073/1‑81 (MQ=255)                                                          tCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTg                   >  1:222088/1‑81 (MQ=255)                                                           cTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTg                   <  1:222046/80‑1 (MQ=255)                                                              cTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTg               <  1:222047/81‑1 (MQ=255)                                                                aTCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGtt             >  1:222081/1‑81 (MQ=255)                                                                     gcgcGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCt        >  2:222082/1‑81 (MQ=255)                                                                      cgcgGCATAAACATACTTCTCTCTATTCCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTg       >  1:222076/1‑81 (MQ=255)                                                                           cATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCtt  <  1:222048/81‑1 (MQ=255)                                                                              |                                                                              AACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTTTCCCTGGGCTT  >  NC_000913/1100221‑1100375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑