Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,101,867	A→G	I87I (ATT→ATC)	csgF ←	curli assembly component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,101,867	0	A	G	100.0%	44.6 / NA	14	I87I (ATT→ATC)	csgF	curli assembly component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/9);  total (5/9)

ATTGACCATCGCGGTTGGCAATATCGACAATATAATCGTTGGTCACCATGCGGCCCGGTTTACCGGTATTAATATTCGACAGTAGCCCACCTAAAATTTGTGACTGGATGGCCTGAGTAAAGTTATCT  >  NC_000913/1101797‑1101924                                                                       |                                                          aTTGACCATCGCGGTTGGCAATATCGACAATATAATCGTTGGTCACCATGCGGCCCGGTTTACCGGTATTGATATTCGa                                                   >  1:222302/1‑79 (MQ=255)    gACCATCGCGGTTGGCAATATCGACAATATAATCGTTGGTCACCATGCGGCCCGGTTTACCGGTATTGATATTCGACAGt                                               <  1:222279/80‑1 (MQ=255)     aCCATCGCGGTTGGCAATATCGACAATATAATCGTTGGTCACCATGCGGCCCGGTTTACCGGTATTGATATTCGACAGTa                                              <  1:222280/80‑1 (MQ=255)     aCCATCGCGGTTGGCAATATCGACAATATAATCGTTGGTCACCATGCGGCCCGGTTTACCGGTATTGATATTCGACAGTAg                                             >  2:222303/1‑81 (MQ=255)      ccATCGCGGTTGGCAATATCGACAATATAATCGTTGGTCACCATGCGGCCCGGTTTACCGGTATTGATATTCGACAGTAGc                                            >  2:222307/1‑81 (MQ=255)        aTCGCGGTTGGCAATATCGACAATATAATCGTTGGTCACCATGCGGCCCGGTTTACCGGTATTGATATTCGACAGTAGccc                                          <  2:222281/81‑1 (MQ=255)             ggTTGGCAATATCGACAATATAATCGTTGGTCACCATGCGGCCCGGTTTACCGGTATTGATATTCGACAGTAGCCCACCTa                                     <  2:222282/81‑1 (MQ=255)                  gCAATATCGACAATATAATCGTTGGTCACCATGCGGCCCGGTTTACCGGTATTGATATTCGACAGTAGCCCACCTAAAAtt                                <  1:222283/81‑1 (MQ=255)                    aaTATCGACAATATAATCGTTGGTCACCATGCGGCCCGGTTTACCGGTATTGATATTCGACAGTAGCCCACCTAAAATTtg                              <  1:222284/81‑1 (MQ=255)                     atatCGACAATATAATCGTTGGTCACCATGCGGCCCGGTTTACCGGTATTGATATTCGACAGTAGCCCACCTAAAATTtgt                             >  1:222306/1‑81 (MQ=255)                          gACAATATAATCGTTGGTCACCATGCGGCCCGGTTTACCGGTATTGATATTCGATAGTAGCCCACCTAAAATTTGTGACTg                        <  1:222285/81‑1 (MQ=255)                                  aaTCGTTGGTCACCATGCGGCCCGGTTTACCGGTATTGATATTCGACAGTAGCCCACCTAAAATTTGTGACTGGATGGCCt                >  1:222304/1‑81 (MQ=255)                                           tCACCATGCGGCCCGGTTTACCGGTATTGATATTCGACAGTAGCCCACCTAAAATTTGTGACTGGATGGCCTGAGTAAAGt       <  1:222286/81‑1 (MQ=255)                                                aTGCGGCCCGGTTTACCGGTATTGATATTCGACAGTAGCCCACCTAAAATTTGTGACTGGATGGCCTGAGTAAAGTTATCt  <  2:222287/81‑1 (MQ=255)                                                                       |                                                          ATTGACCATCGCGGTTGGCAATATCGACAATATAATCGTTGGTCACCATGCGGCCCGGTTTACCGGTATTAATATTCGACAGTAGCCCACCTAAAATTTGTGACTGGATGGCCTGAGTAAAGTTATCT  >  NC_000913/1101797‑1101924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑