Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,105,096	C→T	L46L (CTG→TTG)	csgC →	inhibitor of CsgA amyloid formation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,105,096	0	C	T	100.0%	42.0 / NA	13	L46L (CTG→TTG)	csgC	inhibitor of CsgA amyloid formation
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/11);  total (2/11)

CAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCT  >  NC_000913/1105021‑1105173                                                                            |                                                                              cacggggATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCTTgcgc                                                                          <  2:222625/78‑1 (MQ=255)      ggATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCTTGCGCGAAgg                                                                     >  2:222650/1‑81 (MQ=255)       gATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCTTGCGCGAAGGc                                                                    <  2:222626/81‑1 (MQ=255)         tgtgTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCTTGCGCGAAGGCAg                                                                  <  1:222627/81‑1 (MQ=255)           tgtATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCTTGCGCGAAGGCAGtt                                                                <  2:222628/81‑1 (MQ=255)                                tCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCTTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGc                                           <  1:222629/81‑1 (MQ=255)                                             aTCTTGTCTGTACAGAGTACAAATATTGTCCTTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCt                              >  1:222651/1‑81 (MQ=255)                                                  gTCTGTGCAGAGTACAAATATTGTCCTTGCACGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCa                          <  2:222630/80‑1 (MQ=255)                                                       tgCAGAGTACAAATATTGTCCTTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTg                       <  2:222633/78‑1 (MQ=255)                                                       tgCAGAGTACAAATATTGTCCTTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGcc                     <  1:222631/80‑1 (MQ=255)                                                       tgCAGAGTACAAATATTGTCCTTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGcc                     <  1:222632/80‑1 (MQ=255)                                                            agTACAAATATTGTCCTTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTaa               <  2:222634/81‑1 (MQ=255)                                                                         tCCTTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCt  <  2:222635/81‑1 (MQ=255)                                                                            |                                                                              CAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCT  >  NC_000913/1105021‑1105173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑