Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,114,355	A→G	V379A (GTA→GCA)	mdtG ←	efflux pump MdtG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,114,355	0	A	G	100.0%	107.4 / NA	32	V379A (GTA→GCA)	mdtG	efflux pump MdtG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/19);  total (13/19)

GGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGAT  >  NC_000913/1114278‑1114432                                                                              |                                                                              ggggTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCg                                                                            >  2:224135/1‑81 (MQ=255) ggggTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCg                                                                            >  1:224137/1‑81 (MQ=255)            cgacgacgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCg                                                                 <  2:224105/81‑1 (MQ=255)              acgacgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCg                                                                 >  1:224140/1‑79 (MQ=255)               cgacgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAg                                                              <  2:224106/81‑1 (MQ=255)                gacgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGt                                                             <  1:224107/81‑1 (MQ=255)                 acgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGtt                                                            <  2:224108/81‑1 (MQ=255)                  cgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTc                                                           <  2:224109/81‑1 (MQ=255)                    tAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGc                                                         >  2:224143/1‑81 (MQ=255)                       aCTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTg                                                       >  1:224145/1‑80 (MQ=255)                       aCTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGa                                                      <  2:224110/81‑1 (MQ=255)                               aTGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCgctg                                              >  1:224136/1‑81 (MQ=255)                                  aaTAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTc                                           <  1:224111/81‑1 (MQ=255)                                      gACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAAACGTAGTTCGCTGAAATCGCTGCTCCCAt                                       <  1:224112/81‑1 (MQ=255)                                           cGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAAt                                   <  1:224114/80‑1 (MQ=255)                                           cGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAAt                                   <  1:224113/80‑1 (MQ=255)                                            gTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATgg                                 <  2:224115/81‑1 (MQ=255)                                               gAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTcc                              >  2:224141/1‑81 (MQ=255)                                               gAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTc                               <  2:224116/80‑1 (MQ=255)                                                    acacTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAGTGGTCCGGTa                          <  2:224117/80‑1 (MQ=255)                                                          cGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGtt                     <  1:224118/79‑1 (MQ=255)                                                          cGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTg                    <  2:224119/80‑1 (MQ=255)                                                            ccAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCa                 <  1:224120/81‑1 (MQ=255)                                                              aGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAat               <  1:224121/81‑1 (MQ=255)                                                                   tGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCAc          <  1:224122/81‑1 (MQ=255)                                                                     aCGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGa        <  1:224123/81‑1 (MQ=255)                                                                      cGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGa        >  1:224149/1‑80 (MQ=255)                                                                      cGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGa        >  1:224154/1‑80 (MQ=255)                                                                       gagaAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTTCGGTAACGTTGCCAATATCACGaaa      >  1:224147/1‑81 (MQ=255)                                                                       gagaAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGaaa      >  1:224153/1‑81 (MQ=255)                                                                         gaAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAAc     >  2:224151/1‑80 (MQ=255)                                                                           aaTGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGAt  >  1:224144/1‑81 (MQ=255)                                                                              |                                                                              GGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGAT  >  NC_000913/1114278‑1114432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑