| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,128,283 | G→T | A149S (GCG→TCG) | murJ → | putative lipid II flippase MurJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,128,283 | 0 | G | T | 97.4% | 127.4 / ‑5.7 | 38 | A149S (GCG→TCG) | murJ | putative lipid II flippase MurJ |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (22/15); minor base C (0/1); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTT > NC_000913/1128208‑1128359 | tttGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGAtt < 2:226233/81‑1 (MQ=255)tttGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGAtt > 1:226274/1‑81 (MQ=255) cccTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTg < 2:226234/80‑1 (MQ=255) aCCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACg > 1:226289/1‑81 (MQ=255) aCCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACg > 2:226259/1‑81 (MQ=255) cagccagcTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTg > 1:226278/1‑81 (MQ=255) aaaGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTtctc > 1:226277/1‑81 (MQ=255) aaGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTtct < 1:226235/79‑1 (MQ=255) gATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGAt > 2:226286/1‑81 (MQ=255) ccTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCgg > 1:226276/1‑81 (MQ=255) ccTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCgg > 2:226279/1‑81 (MQ=255) ccTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCg < 2:226236/80‑1 (MQ=255) tCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGt < 2:226237/80‑1 (MQ=255) ccTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGttt < 1:226239/80‑1 (MQ=255) ccTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGttt < 2:226238/80‑1 (MQ=255) ccTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTg > 2:226271/1‑81 (MQ=255) cTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGttttc < 1:226240/81‑3 (MQ=255) atatCTTGCTGATCTCCCTAGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTc > 1:226297/1‑81 (MQ=255) tatCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTc > 2:226293/1‑80 (MQ=255) tatCTTGCTGATCTCCCTGGCGTCGCTGGTGGGACCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCt < 1:226241/79‑1 (MQ=255) ttGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAaca > 2:226295/1‑81 (MQ=255) gCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACt > 2:226285/1‑81 (MQ=255) gCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACt > 2:226283/1‑81 (MQ=255) gATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCt > 2:226287/1‑81 (MQ=255) gtctcCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCt < 2:226242/79‑1 (MQ=255) cccTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACa > 2:226284/1‑81 (MQ=255) cccTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACa > 1:226288/1‑81 (MQ=255) ccTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCtt < 2:226245/76‑1 (MQ=255) ccTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCtt < 2:226243/76‑1 (MQ=255) ccTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTaa < 2:226244/78‑1 (MQ=255) ccTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTa < 1:226246/77‑1 (MQ=255) ccTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACAt < 1:226247/81‑1 (MQ=255) gCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGc < 2:226248/81‑1 (MQ=255) gTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCAt > 2:226280/1‑81 (MQ=255) cGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGa > 2:226291/1‑81 (MQ=255) gCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGAt > 2:226294/1‑81 (MQ=255) gtggGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGt > 1:226282/1‑81 (MQ=255) gggATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGttt > 2:226298/1‑81 (MQ=255) | TTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTT > NC_000913/1128208‑1128359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |