Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,131,502	A→G	N22S (AAC→AGC)	flgC →	flagellar basal‑body rod protein FlgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,131,502	0	A	G	100.0%	90.6 / NA	27	N22S (AAC→AGC)	flgC	flagellar basal‑body rod protein FlgC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/14);  total (13/14)

CGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAA  >  NC_000913/1131425‑1131572                                                                              |                                                                       cGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGt                                                                     <  2:226810/81‑1 (MQ=255)  ggAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTg                                                                    >  1:226843/1‑81 (MQ=255)      aTTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTggcgg                                                                >  2:226840/1‑81 (MQ=255)        tAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTggcggc                                                               >  2:226842/1‑80 (MQ=255)          aCGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCa                                                             <  2:226811/80‑1 (MQ=255)                   aCTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGCCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGc                                                   <  2:226812/81‑1 (MQ=255)                       ctgAATATTTTTGATATCGCCGGGTCAGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAAt                                               <  1:226813/81‑1 (MQ=255)                          aaTATTTTTGATATCGCCGGGTTGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATg                                              <  1:226814/79‑1 (MQ=255)                                   gATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGt                                    >  1:226845/1‑80 (MQ=255)                                   gATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGt                                    >  2:226854/1‑80 (MQ=255)                                   gATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGt                                    <  1:226815/80‑1 (MQ=255)                                        cGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCgg                              >  1:226850/1‑81 (MQ=255)                                         gCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTTATAGCGTGACCGGt                             >  1:226860/1‑81 (MQ=255)                                            gggTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTccc                          >  2:226841/1‑81 (MQ=255)                                             ggTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCg                         <  1:226816/81‑1 (MQ=255)                                               tCGGCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGAt                       <  1:226817/81‑1 (MQ=255)                                                 ggCGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATgg                     <  1:226818/81‑1 (MQ=255)                                                   cGTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGAc                   <  2:226819/81‑1 (MQ=255)                                                    gTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGa                    >  2:226858/1‑79 (MQ=255)                                                    gTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGa                    >  2:226857/1‑79 (MQ=255)                                                    gTTAACTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGa                    >  2:226847/1‑79 (MQ=255)                                                         cTGCCCAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCat             <  1:226820/81‑1 (MQ=255)                                                            cccAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCatat           <  1:226822/80‑1 (MQ=255)                                                            cccAGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATc          <  2:226821/81‑1 (MQ=255)                                                               aGTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCggg       >  1:226848/1‑81 (MQ=255)                                                                gTCCCAGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGc      <  2:226823/81‑1 (MQ=255)                                                                     aGCGCCTGAGCGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCaaaa  >  2:226852/1‑80 (MQ=255)                                                                              |                                                                       CGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAA  >  NC_000913/1131425‑1131572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑