Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,135,222	C→T	A165V (GCA→GTA)	flgG →	flagellar basal‑body rod protein FlgG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,135,222	0	C	T	100.0%	94.6 / NA	27	A165V (GCA→GTA)	flgG	flagellar basal‑body rod protein FlgG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/15);  total (12/15)

GATCACCATTCCGGCGAATGCGTTAAGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGCAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACC  >  NC_000913/1135145‑1135299                                                                              |                                                                              gATCACCATTCCGGCGAATGCGTTAAGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGc                                                                            >  2:227551/1‑81 (MQ=255) gATCACCATTCCGGCGAATGCGTTAAGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGc                                                                            >  2:227549/1‑81 (MQ=255)  aTCACCATTCCGGCGAATGCGTTAAGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCt                                                                           <  2:227512/81‑1 (MQ=255)   tCACCATTCCGGCGAATGCGTTAAGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTc                                                                          <  1:227513/81‑1 (MQ=255)     aCCATTCCGGCGAATGCGTTAAGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTcc                                                                         <  1:227514/80‑1 (MQ=255)       cATTCCGGCGAATGCGTTAAGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGt                                                                      <  1:227515/81‑1 (MQ=255)            cGGCGAATGCGTTAAGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGTTCAg                                                                  <  1:227516/80‑1 (MQ=255)                gAATGCGTTAAGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTgg                                                             >  2:227543/1‑81 (MQ=255)                 aaTGCGTTAAGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTggg                                                            >  2:227555/1‑81 (MQ=255)                     cGTTAAGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGc                                                        >  2:227545/1‑81 (MQ=255)                         aaGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCa                                                     <  2:227518/80‑1 (MQ=255)                         aaGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCa                                                     <  2:227517/80‑1 (MQ=255)                           gTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCAAtc                                                  >  2:227544/1‑81 (MQ=255)                              tCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCAAtctc                                                <  1:227519/80‑1 (MQ=255)                                aCCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCAATCTcacc                                             <  1:227520/81‑1 (MQ=255)                                      ggTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCtt                                        <  2:227521/80‑1 (MQ=255)                                             aTGGCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTatgaatg                                <  2:227522/81‑1 (MQ=255)                                               ggCGTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTatgaatga                               >  2:227562/1‑80 (MQ=255)                                                  gTGGTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTATGAATGACAcc                           >  1:227556/1‑81 (MQ=255)                                                     gTCAGCGTAACCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTATGAATGACACCgg                         <  1:227523/80‑1 (MQ=255)                                                           gTAACCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTATGAATGACACCGGGCTg                     >  2:227546/1‑78 (MQ=255)                                                             aaCCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTATGAATGACACCGGGCTGGagag                >  2:227548/1‑81 (MQ=255)                                                              aCCCAACAAGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTATGAATGACACCGGGCTGGAGAGc               <  2:227524/81‑1 (MQ=255)                                                                     aaGGCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCg        <  1:227525/81‑1 (MQ=255)                                                                        gCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGaaa     <  2:227526/81‑1 (MQ=255)                                                                        gCCAGGTAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGaa      >  1:227550/1‑80 (MQ=255)                                                                           aGGTAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAAcc  >  1:227559/1‑81 (MQ=255)                                                                              |                                                                              GATCACCATTCCGGCGAATGCGTTAAGTATCACCATCGGTCGTGATGGCGTGGTCAGCGTAACCCAACAAGGCCAGGCAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACC  >  NC_000913/1135145‑1135299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑