Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,146,195	T→C	E134G (GAG→GGG)	yceF ←	m(7)GTP pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,146,195	0	T	C	100.0%	110.0 / NA	32	E134G (GAG→GGG)	yceF	m(7)GTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/15);  total (17/15)

AGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAAC  >  NC_000913/1146117‑1146272                                                                               |                                                                              aGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATcccc                                                                             <  1:229548/81‑1 (MQ=255) aGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATcccc                                                                             <  1:229547/81‑1 (MQ=255)     tAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCt                                                                         <  2:229550/81‑1 (MQ=255)     tAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCt                                                                         <  2:229549/81‑1 (MQ=255)      aaTGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTc                                                                        >  2:229587/1‑81 (MQ=255)       aTGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCg                                                                       >  1:229578/1‑81 (MQ=255)       aTGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCg                                                                       >  1:229579/1‑81 (MQ=255)        tGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGc                                                                      >  1:229585/1‑81 (MQ=255)                ccTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGa                                                              >  2:229594/1‑81 (MQ=255)                 cTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGAc                                                             <  1:229551/81‑1 (MQ=255)                    cACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACg                                                            <  2:229552/79‑1 (MQ=255)                               cTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTc                                                >  1:229589/1‑80 (MQ=255)                                 aCCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCaa                                              >  2:229583/1‑80 (MQ=255)                                  cccGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCaaa                                             <  1:229553/80‑1 (MQ=255)                                  cccGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCaa                                              >  2:229590/1‑79 (MQ=255)                                    cgcgCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAgg                                          >  2:229596/1‑81 (MQ=255)                                     gcgcAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGc                                         >  1:229593/1‑81 (MQ=255)                                       gcAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTc                                       <  2:229554/81‑1 (MQ=255)                                       gcAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTc                                       >  2:229572/1‑81 (MQ=255)                                             cAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTc                                 <  1:229555/81‑1 (MQ=255)                                              aGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCt                                >  2:229600/1‑81 (MQ=255)                                              aGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCt                                >  2:229602/1‑81 (MQ=255)                                                         tttACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGa                       <  1:229556/79‑1 (MQ=255)                                                            aCGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGcc                   >  1:229598/1‑80 (MQ=255)                                                                  gTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGc            <  1:229557/81‑1 (MQ=255)                                                                        aTCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAAt       >  2:229604/1‑80 (MQ=255)                                                                        aTCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAAt       <  2:229558/80‑1 (MQ=255)                                                                        aTCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAAt       <  1:229559/80‑1 (MQ=255)                                                                         tCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAAtt      <  2:229560/80‑1 (MQ=255)                                                                           aaTCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTaaa   >  1:470733/1‑81 (MQ=255)                                                                           aaTCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTaaa   >  1:229601/1‑81 (MQ=255)                                                                            aTCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAaac  <  2:229562/81‑1 (MQ=255)                                                                            aTCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAaac  <  1:229563/81‑1 (MQ=255)                                                                            aTCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAaac  <  1:229561/81‑1 (MQ=255)                                                                               |                                                                              AGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAAC  >  NC_000913/1146117‑1146272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑