Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,150,676	T→C	F3L (TTT→CTT)	fabG →	3‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,150,676	0	T	C	100.0%	91.0 / NA	27	F3L (TTT→CTT)	fabG	3‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/13);  total (14/13)

CTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCG  >  NC_000913/1150601‑1150752                                                                            |                                                                             cTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGaa                                                                         <  1:230361/81‑1 (MQ=255)   gACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAg                                                                        >  2:230387/1‑80 (MQ=255)    aCCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGa                                                                      <  2:230362/81‑1 (MQ=255)     ccGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGaa                                                                     <  2:230363/81‑1 (MQ=255)       gCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGaaaa                                                                   <  1:230364/81‑1 (MQ=255)         cTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAAt                                                                 <  1:230365/81‑1 (MQ=255)            ggCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATc                                                                <  1:230366/79‑1 (MQ=255)              cgcTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCAc                                                            >  2:230379/1‑81 (MQ=255)                  gaacgaacCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTgg                                                         >  1:230400/1‑80 (MQ=255)                   aacgaacCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTa                                                       <  1:230367/81‑1 (MQ=255)                     cgaacCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAAc                                                     <  1:230368/81‑1 (MQ=255)                                 gATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCcg                                         >  1:230399/1‑81 (MQ=255)                                  aTGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCcgc                                        <  1:230369/81‑1 (MQ=255)                                  aTGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCcgc                                        >  2:230390/1‑81 (MQ=255)                                          ggcgCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTgg                                >  1:230402/1‑81 (MQ=255)                                           gcgcTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGc                               >  2:230393/1‑81 (MQ=255)                                             gcTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCcg                             >  1:230391/1‑81 (MQ=255)                                             gcTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCcg                             >  2:230410/1‑81 (MQ=255)                                              cTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCcgc                            >  2:230395/1‑81 (MQ=255)                                                  aGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCaa                        >  1:230403/1‑81 (MQ=255)                                                  aGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCaa                        >  1:230398/1‑81 (MQ=255)                                                    cTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAAtt                      >  1:230396/1‑81 (MQ=255)                                                            gagGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCANTTGCTGAAAc              <  1:230370/81‑1 (MQ=255)                                                                aaaaTCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTc          <  2:230371/81‑1 (MQ=255)                                                                   aTCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCa       <  2:230372/81‑1 (MQ=255)                                                                    tCATGAATCTTGAAGGAAAAATCGCACTGGTGACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAg      <  2:230373/81‑1 (MQ=255)                                                                        gAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCg  >  2:230401/1‑81 (MQ=255)                                                                            |                                                                             CTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCG  >  NC_000913/1150601‑1150752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑