Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,163,982	G→A	M241I (ATG→ATA)	thiK →	thiamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,163,982	0	G	A	100.0%	141.2 / NA	39	M241I (ATG→ATA)	thiK	thiamine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/20);  total (19/20)

TGCCACTCGCGCGAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATGCTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGCCGATGACACCTG  >  NC_000913/1163910‑1164059                                                                         |                                                                              tGCCACTCGCGCGAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGc                                                                       <  2:232635/81‑1 (MQ=255)   ccACTCGCGCGAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCa                                                                      <  1:232636/80‑1 (MQ=255)     aCTCGCGCGAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAggg                                                                   >  1:232685/1‑81 (MQ=255)     aCTCGCGCGAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAggg                                                                   >  1:232674/1‑81 (MQ=255)      cTCGCGCGAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGggt                                                                  <  2:232637/81‑1 (MQ=255)         gcgcgAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGggtggt                                                               >  1:232669/1‑81 (MQ=255)             gAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGttt                                                             >  1:232672/1‑79 (MQ=255)              aaGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAg                                                          >  1:232679/1‑81 (MQ=255)                  tttATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTAcc                                                      <  2:232638/81‑1 (MQ=255)                    tATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCg                                                     <  1:232639/80‑1 (MQ=255)                    tATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGc                                                    >  1:232677/1‑81 (MQ=255)                      tCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTg                                                  >  1:232678/1‑81 (MQ=255)                      tCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTg                                                  <  2:232640/81‑1 (MQ=255)                        cGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTgg                                                 <  2:232641/80‑1 (MQ=255)                         ggCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCg                                               <  1:232642/81‑1 (MQ=255)                             cAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACa                                            >  1:232693/1‑80 (MQ=255)                                   tGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGc                                     >  1:232686/1‑81 (MQ=255)                                     gCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCg                                    <  2:232643/80‑1 (MQ=255)                                      cGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGAt                                  <  2:232644/81‑1 (MQ=255)                                       gtcaggtcagGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGAt                                  >  1:232682/1‑80 (MQ=255)                                       gtcaggtcagGCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATc                                 <  2:232645/81‑1 (MQ=255)                                          aggtcaggCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATcaa                               >  2:232676/1‑80 (MQ=255)                                             tcaggCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAAt                           <  1:232646/81‑1 (MQ=255)                                               aggCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAAtt                          >  2:232690/1‑80 (MQ=255)                                                 gCGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTAt                       <  2:232647/81‑1 (MQ=255)                                                  cGATGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATc                      <  2:232648/81‑1 (MQ=255)                                                    aTGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAg                    <  1:232649/81‑1 (MQ=255)                                                     tGGTTTCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAgg                   >  2:232683/1‑81 (MQ=255)                                                        tttCCCTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTg                <  1:232650/81‑1 (MQ=255)                                                           cccTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGcc             >  1:232692/1‑81 (MQ=255)                                                            ccTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGcc             >  1:232703/1‑80 (MQ=255)                                                            ccTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGcc             <  2:232651/80‑1 (MQ=255)                                                             cTGGCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGCCGa           <  1:232652/81‑1 (MQ=255)                                                               ggCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGCCGAt          >  2:232673/1‑80 (MQ=255)                                                               ggCTGCTGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGCCGAt          >  2:232688/1‑80 (MQ=255)                                                                gctgctGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGCCGAt          <  2:232653/79‑1 (MQ=255)                                                                gctgctGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGCCGATg         <  2:232654/80‑1 (MQ=255)                                                                gctgctGATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGCCGATGa        >  2:232689/1‑81 (MQ=255)                                                                      gATACTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGCCGATGACACCTg  >  1:232691/1‑81 (MQ=255)                                                                         |                                                                              TGCCACTCGCGCGAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTGATGCTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGCCGATGACACCTG  >  NC_000913/1163910‑1164059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑