Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,182,497	A→T	D111E (GAT→GAA)	potD ←	spermidine preferential ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,182,497	0	A	T	100.0%	80.9 / NA	23	D111E (GAT→GAA)	potD	spermidine preferential ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (8/15);  total (8/15)

ACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAAC  >  NC_000913/1182419‑1182571                                                                               |                                                                           acCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTc                                                                          >  2:235671/1‑81 (MQ=255) acCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTc                                                                          <  2:235642/81‑1 (MQ=255)    aaTCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTCgag                                                                       <  2:235643/81‑1 (MQ=255)      tCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTCGAGAt                                                                     <  1:235644/81‑1 (MQ=255)                  cccAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTCGAGATTGCTGAAGTTTg                                                         >  2:235667/1‑81 (MQ=255)                   ccAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTCGAGATTGCTGAAGTTTGt                                                        <  2:235645/81‑1 (MQ=255)                       atatACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTCGAGATTGCTGAAGTTTGTTAAc                                                    <  1:235646/81‑1 (MQ=255)                         ataCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTCGAGATTGCTGAAGTTTGTTAACtt                                                  >  1:235666/1‑81 (MQ=255)                          taCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTCGAGATTGCTGAAGTTTGTTAACtt                                                  <  1:235647/80‑1 (MQ=255)                          taCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTCGAGATTGCTGAAGTTTGTTAACTTc                                                 <  2:235648/81‑1 (MQ=255)                           aCGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTCGAGATTGCTGAAGTTTGTTAACTTc                                                 >  1:235649/1‑80 (MQ=255)                               aaTGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTCGAGATTGCTGAAGTTTGTTAACTTCGACtt                                            <  1:235650/81‑1 (MQ=255)                                aTGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTCGAGATTGCTGAAGTTTGTTAACTTCGACTTg                                           >  1:235675/1‑81 (MQ=255)                                   gAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTc                                         <  1:235651/80‑1 (MQ=255)                                    agtagtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGa                                       <  2:235652/81‑1 (MQ=255)                                      tagtCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGTTCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGAtc                                     <  1:235653/81‑1 (MQ=255)                                                         aaGGCTTGTTGAGCATGTCTGGTTCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCcttctt                  <  2:235654/81‑1 (MQ=255)                                                                gttgAGCATGTCTGGTTCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGc             <  1:235655/79‑1 (MQ=255)                                                                gttgAGCATGTCTGGTTCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCAt           >  2:235668/1‑81 (MQ=255)                                                                    aGCATGTCTGGTTCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTa       >  1:235725/1‑81 (MQ=255)                                                                      cATGTCTGGTTCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATc     <  1:235656/81‑1 (MQ=255)                                                                       aTGTCTGGTTCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATc     >  1:235669/1‑80 (MQ=255)                                                                          tCTGGTTCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAAc  <  1:235657/80‑1 (MQ=255)                                                                               |                                                                           ACCAATCGCCGTCGCACCCCAGATATACGGAATGGAGTAGTCGTTATTCGGGTCAAAAGGCTTGTTGAGCATGTCTGGATCGAGATTGCTGAAGTTTGTTAACTTCGACTTGTCGATCTTCTGGATCATCCCTTCTTTACGCATTTTATCAAC  >  NC_000913/1182419‑1182571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑