Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,191,441	T→C	K182K (AAA→AAG)	purB ←	adenylosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,191,441	0	T	C	100.0%	87.1 / NA	26	K182K (AAA→AAG)	purB	adenylosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/15);  total (11/15)

GATTTTGCCGAGGATCTCCACCTGGTTAAGCTGGCGGTACTGGCGCTCCATACGGTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGC  >  NC_000913/1191366‑1191515                                                                            |                                                                           gATTTTGCCGAGGATCTCCACCTGGTTAAGCTGGCGGTACTGGCGCTCCATACGGTAGGCGACGTTTGCCATCTCCTTAc                                                                        <  1:237165/80‑1 (MQ=255) gATTTTGCCGAGGATCTCCACCTGGTTAAGCTGGCGGTACTGGCGCTCCATACGGTAGGCGACGTTTGCCATCTCCTTAc                                                                        <  2:237166/80‑1 (MQ=255)     ttGCCGAGGATCTCCACCTGGTTAAGCTGGCGGTACTGGCGCTCCATACGGTAGGCGACGTTTGCCATCTCCTTACCGAt                                                                    <  1:237167/80‑1 (MQ=255)            ggATCTCCACCTGGTTAAGCTGGCGGTACTGGCGCTCCATACGGTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACg                                                            <  2:237168/81‑1 (MQ=255)               tctcCACCTGGTTAAGCTGGCGGTACTGGCGCTCCATACGGTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCg                                                         >  2:237196/1‑81 (MQ=255)                ctcCACCTGGTTAAGCTGGCGGTACTGGCGCTCCATACGGTAGGCGACGCTTGCCATCTCCTTACCGATGGTTGACGGCGt                                                        >  2:237204/1‑81 (MQ=255)                     ccTGGTTAAGCTGGCGGTACTGGCGCTCCATACGGTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGtggctg                                                   >  1:237210/1‑81 (MQ=255)                                       aCTGGCGCTCCATACGGTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTAc                                    >  2:237200/1‑78 (MQ=255)                                        cTGGCGCTCCATACGGTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACgaga                                >  1:237193/1‑81 (MQ=255)                                         tGGCGCTCCATACGGTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACgaga                                >  2:237202/1‑80 (MQ=255)                                          ggCGCTCCATACGGTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACgaga                                >  2:237212/1‑79 (MQ=255)                                          ggCGCTCCATACGGTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACgaga                                >  1:237205/1‑79 (MQ=255)                                           gcgcTCCATACGGTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGAcag                             <  2:237169/81‑1 (MQ=255)                                             gcTCCATACGGTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGAcagca                           <  2:237170/81‑1 (MQ=255)                                                  aTACGGTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCggg                      <  1:237171/81‑1 (MQ=255)                                                    aCGGTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGa                     >  2:237198/1‑80 (MQ=255)                                                     cGGTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGat                    <  2:237172/80‑1 (MQ=255)                                                      ggTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGTAGCGGGatat                  <  2:237173/81‑1 (MQ=255)                                                       gTAGGCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCCGACCGTGGGTACGAGACAGCAGCGGGATATc                 >  2:237203/1‑81 (MQ=255)                                                          ggCGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATcgc               <  2:237174/80‑1 (MQ=255)                                                            cGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGa             >  1:237206/1‑80 (MQ=255)                                                            cGACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGAt            <  2:237175/81‑1 (MQ=255)                                                             gACGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATa           <  2:237176/81‑1 (MQ=255)                                                               cGTTTGCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACt         <  1:237177/81‑1 (MQ=255)                                                                    gCCATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAAc     <  2:237178/80‑1 (MQ=255)                                                                      cATCTCCTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGc  <  1:237179/81‑1 (MQ=255)                                                                         ctccTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGc  >  2:237201/1‑78 (MQ=255)                                                                         ctccTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGc  >  1:237223/1‑78 (MQ=255)                                                                            |                                                                           GATTTTGCCGAGGATCTCCACCTGGTTAAGCTGGCGGTACTGGCGCTCCATACGGTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGC  >  NC_000913/1191366‑1191515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑