Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	19,070	T→C	V119A (GTA→GCA)	nhaR →	DNA‑binding transcriptional activator NhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	19,070	0	T	C	100.0%	99.7 / NA	29	V119A (GTA→GCA)	nhaR	DNA‑binding transcriptional activator NhaR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/18);  total (11/18)

CAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAAC  >  NC_000913/18993‑19147                                                                              |                                                                              cAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAg                                                                             >  2:4475/1‑80 (MQ=255) cAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAg                                                                             <  1:4434/80‑1 (MQ=255)   aTTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAg                                                                          >  1:4470/1‑81 (MQ=255)      tATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGc                                                                        <  2:4435/80‑1 (MQ=255)           tttGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGcc                                                                   <  1:4436/80‑1 (MQ=255)           tttGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGcc                                                                   <  1:4437/80‑1 (MQ=255)            ttGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCa                                                                 >  2:4492/1‑81 (MQ=255)             tGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCAt                                                                >  2:4484/1‑81 (MQ=255)              gACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCAtt                                                               >  1:4521/1‑81 (MQ=255)                  ttGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATc                                                           <  1:4438/81‑1 (MQ=255)                       gTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCgc                                                      >  1:4483/1‑81 (MQ=255)                       gTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCgc                                                      >  2:4491/1‑81 (MQ=255)                                 cACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGaa                                             <  1:4439/80‑1 (MQ=255)                                   cTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATcc                                          <  1:4440/81‑1 (MQ=255)                                        cAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATccaccca                                     >  1:4490/1‑81 (MQ=255)                                          aaCGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACg                                   <  2:4441/81‑1 (MQ=255)                                           aCGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGa                                  <  2:4442/81‑1 (MQ=255)                                            cGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGaa                                 <  2:4443/81‑1 (MQ=255)                                               cTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAAtg                              <  2:4444/81‑1 (MQ=255)                                                  gTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAAtgctg                           <  1:4445/81‑1 (MQ=255)                                                     aGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGctgctg                        >  2:4487/1‑81 (MQ=255)                                                           gTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCaa                  <  1:4446/81‑1 (MQ=255)                                                                 aaCGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTaa              <  1:4448/79‑1 (MQ=255)                                                                 aaCGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTaa              <  1:4447/79‑1 (MQ=255)                                                                  aCGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGt            <  1:4449/80‑1 (MQ=255)                                                                     ccgcAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGc        <  1:4450/81‑1 (MQ=255)                                                                       gcAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCa       >  2:4498/1‑80 (MQ=255)                                                                       gcAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCAt      <  1:4451/81‑1 (MQ=255)                                                                           tGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAAc  >  2:4495/1‑81 (MQ=255)                                                                              |                                                                              CAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAAC  >  NC_000913/18993‑19147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑