Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,230,433	C→T	intergenic (‑39/‑334)	hlyE ← / → umuD	hemolysin E/DNA polymerase V protein UmuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,230,433	0	C	T	100.0%	19.3 / NA	7	intergenic (‑39/‑334)	hlyE/umuD	hemolysin E/DNA polymerase V protein UmuD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/2);  total (5/2)

CTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTC  >  NC_000913/1230364‑1230499                                                                      |                                                                   cTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTAGCCTCTTTAAATATATAAATTGTAATGAAATTCCTGTTTTa                                                          >  2:242271/1‑80 (MQ=255)    ccGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAATTCCTGTTTTACAAc                                                      >  2:242277/1‑81 (MQ=255)        tttATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAATTCCTGTTTTACAACTAt                                                   >  2:242279/1‑80 (MQ=255)                    gATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAATTCCTGTTTTACAACTATTAATAAATTTTAc                                      >  2:242281/1‑81 (MQ=255)                           gTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAATTCCTGTTTTACAACTATTAATAAATTTTACTTCATCt                               <  1:242267/81‑1 (MQ=255)                                                         aaTTGTAATGAAATTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTc  >  1:242283/1‑80 (MQ=255)                                                         aaTTGTAATGAAATTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTc  <  2:242268/80‑1 (MQ=255)                                                                      |                                                                   CTACCGTTTTATCTGCAACGATTTCAGTCATAATCATTCGCCTCTTTAAATATATAAATTGTAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTC  >  NC_000913/1230364‑1230499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑