Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,242,600	C→T	G161R (GGG→AGG)	ldcA ←	murein L,D‑carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,242,600	0	C	T	100.0%	129.9 / NA	36	G161R (GGG→AGG)	ldcA	murein L,D‑carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/17);  total (19/17)

CCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCC  >  NC_000913/1242527‑1242676                                                                          |                                                                             ccGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCAt                                                                       >  1:244204/1‑81 (MQ=255)   gATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTc                                                                     <  2:244168/81‑1 (MQ=255)    aTCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTc                                                                     >  2:244218/1‑80 (MQ=255)      cAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAAt                                                                  >  1:244203/1‑81 (MQ=255)       aGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGCCCTTCCCTTTGCCATTCAATg                                                                 >  1:244207/1‑81 (MQ=255)        gTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATgg                                                                >  2:244221/1‑81 (MQ=255)             aTCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAg                                                           <  1:244169/81‑1 (MQ=255)                aGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGtt                                                        <  2:244170/81‑1 (MQ=255)                    tCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCAt                                                    >  1:244220/1‑81 (MQ=255)                     cGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCAtt                                                   >  1:244215/1‑81 (MQ=255)                      gCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTg                                                  <  2:244171/81‑1 (MQ=255)                        aaGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCg                                                <  1:244172/81‑1 (MQ=255)                          gATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGt                                               >  2:244213/1‑80 (MQ=255)                          gATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTa                                              <  1:244173/81‑1 (MQ=255)                            ttGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAAc                                            >  1:244210/1‑81 (MQ=255)                             tGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACg                                           >  2:244226/1‑81 (MQ=255)                             tGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACg                                           >  1:244211/1‑81 (MQ=255)                               ccTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACgcc                                         <  1:244174/81‑1 (MQ=255)                                cTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACgc                                          >  2:244195/1‑79 (MQ=255)                                 tCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACgccag                                       >  1:244188/1‑81 (MQ=255)                                  ccccACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACgccagc                                      >  2:244228/1‑81 (MQ=255)                                  ccccACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACgccagc                                      >  1:244217/1‑81 (MQ=255)                                     cacaACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACgccagc                                      >  2:244225/1‑78 (MQ=255)                                     cacaACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGccagccag                                   <  1:244175/81‑1 (MQ=255)                                          cGTGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATg                              <  2:244176/81‑1 (MQ=255)                                           gTGCCTTCGGCTCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATgg                             <  2:244177/81‑1 (MQ=255)                                            tGCCTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGt                            <  2:244178/81‑1 (MQ=255)                                              ccTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGc                          <  1:244179/81‑1 (MQ=255)                                               cTTCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCt                         <  1:244180/81‑1 (MQ=255)                                                ttCGGCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTc                        >  1:244222/1‑81 (MQ=255)                                                    gCCCGACATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTa                    <  2:244181/81‑1 (MQ=255)                                                      ccaaCATGTCGGGCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTaaa                  <  2:244182/78‑1 (MQ=255)                                                                  gCCTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCgttcagttc      <  2:244183/81‑1 (MQ=255)                                                                   ccTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCgttcagttca     <  1:244184/81‑1 (MQ=255)                                                                    cTTCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCAt    >  1:244230/1‑81 (MQ=255)                                                                      tCCCTTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATcc  >  2:244234/1‑81 (MQ=255)                                                                          |                                                                             CCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCC  >  NC_000913/1242527‑1242676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑