Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,245,163	A→G	I2V (ATT→GTT)	ycgY →	uncharacterized protein YcgY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,163	0	A	G	100.0%	48.3 / NA	15	I2V (ATT→GTT)	ycgY	uncharacterized protein YcgY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/9);  total (6/9)

CGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGC  >  NC_000913/1245087‑1245239                                                                             |                                                                             cGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTc                                                                           <  1:244555/80‑1 (MQ=255)       ccGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTCAGaaaa                                                                     >  1:244556/1‑80 (MQ=255)          aTAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTCAGAaaataaa                                                                 >  2:244577/1‑81 (MQ=255)             aaGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTCAGAAAATAAatat                                                              <  1:244557/81‑1 (MQ=255)                tCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTCAGAAAATAAATAtttt                                                           <  2:244558/81‑1 (MQ=255)                 cGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTCAGAAAATAAATAttt                                                            >  2:244578/1‑79 (MQ=255)                      cccGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTCAGAAAATAAATATTTTAGACa                                                      <  1:244559/80‑1 (MQ=255)                                acaacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTCAGAAAATAAATATTTTAGACAGTAGCCAGt                                             >  2:244582/1‑79 (MQ=255)                                      gAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGtt                                     <  1:244560/81‑1 (MQ=255)                                      gAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGtt                                     <  2:244561/81‑1 (MQ=255)                                           cTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGGTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTaaaa                                 <  2:244562/80‑1 (MQ=255)                                                        tGTTTCGTTGTTTTTGGTGTGGTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGt                   >  2:244583/1‑81 (MQ=255)                                                         gTTTCGTTGTTTTTGGTGTGGTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGt                   >  2:244579/1‑80 (MQ=255)                                                                    tttGGTGTGGTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGtttt        <  1:244563/80‑1 (MQ=255)                                                                         tgtgGTTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTgc  <  1:244564/81‑1 (MQ=255)                                                                             |                                                                             CGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGC  >  NC_000913/1245087‑1245239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑