| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,250,348 | G→T | A164E (GCG→GAG) | dhaK ← | dihydroxyacetone kinase subunit K |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,250,348 | 0 | G | T | 96.0% | 83.2 / ‑3.6 | 25 | A164E (GCG→GAG) | dhaK | dihydroxyacetone kinase subunit K |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (8/16); total (8/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
GCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTT > NC_000913/1250270‑1250423 | gCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCtcctcc < 2:245363/81‑1 (MQ=255) ccTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTgc < 1:245365/80‑1 (MQ=255) ccTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTgc < 1:245364/80‑1 (MQ=255) tGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTAc > 2:245406/1‑81 (MQ=255) aGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACg < 1:245366/80‑1 (MQ=255) gTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAg > 1:245407/1‑81 (MQ=255) gCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGttt < 1:245367/81‑1 (MQ=255) gATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTc < 2:245368/79‑1 (MQ=255) gATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTc < 2:245369/79‑1 (MQ=255) attattCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTCAAt < 1:245370/81‑1 (MQ=255) ttattCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTCAAtt < 2:245371/81‑1 (MQ=255) ttCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTCAATTaa < 1:245372/80‑1 (MQ=255) ttCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTCAATTaa < 1:245373/80‑1 (MQ=255) gCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTgg > 2:245408/1‑81 (MQ=255) ttCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAAcgcc < 1:245374/81‑1 (MQ=255) ccGCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAAcgccg < 1:245375/80‑1 (MQ=255) gCACAGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGt < 2:245376/81‑1 (MQ=255) cacaGGCGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTc > 1:245414/1‑81 (MQ=255) caGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGc < 2:245377/81‑1 (MQ=255) cGTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCgg > 1:245421/1‑81 (MQ=255) gTCCAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGc < 2:245378/81‑1 (MQ=255) cAGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGt < 1:245379/81‑1 (MQ=255) aGTGAGTCGCCACGCTCCTCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGta < 1:245380/81‑1 (MQ=255) cACGCTCCTCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGt > 2:245412/1‑81 (MQ=255) cACGCTCCTCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGt > 2:245423/1‑81 (MQ=255) gCTCCTCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCTCCCGGCAGTATAAAGACTGTCtt > 1:245453/1‑81 (MQ=255) | GCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTT > NC_000913/1250270‑1250423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |