Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,253,815	C→T	G713D (GGC→GAC)	ycgV ←	putative autotransporter adhesin YcgV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,253,815	0	C	T	100.0%	76.9 / NA	22	G713D (GGC→GAC)	ycgV	putative autotransporter adhesin YcgV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/9);  total (13/9)

CATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTG  >  NC_000913/1253744‑1253891                                                                        |                                                                             cATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGTCGCTCAGtt                                                                     <  2:245886/81‑1 (MQ=255) cATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGTCGCTCAGtt                                                                     >  1:245906/1‑81 (MQ=255)    aCAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGTCGCTCAGTTTGc                                                                  >  1:245913/1‑81 (MQ=255)      aaCGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGTCGCTCAGTTTGCCa                                                                >  1:245905/1‑81 (MQ=255)      aaCGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGTCGCTCAGTTTGCCa                                                                >  1:245920/1‑81 (MQ=255)       aCGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGTCGCTCAGTTTGCCAc                                                               >  1:245909/1‑81 (MQ=255)               aCATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGTCGCTCAGTTTGCCACTGGCAAAg                                                       >  1:245911/1‑81 (MQ=255)                   cAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGTCGCTCAGTTTGCCACTGGCAAAGGAGt                                                   >  1:245910/1‑81 (MQ=255)                         gaCGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGTCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGc                                             >  1:245908/1‑81 (MQ=255)                            gTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGTCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTc                                          <  1:245887/81‑1 (MQ=255)                             tttATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGTCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTcc                                         <  1:245888/81‑1 (MQ=255)                                aTCCCCACCAAACCGGATACCGCTGTAACCCATGTCAAAGTCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTcccc                                       <  1:245889/80‑1 (MQ=255)                                          aaCTGGATACCGCTGTAACCCATGTCAAAGTCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTgcg                             >  2:245919/1‑80 (MQ=255)                                                    cGCTGTAACCCATGTCAAAGTCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGatat                  >  1:245916/1‑81 (MQ=255)                                                     gCTGTAACCCATGTCAAAGTCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATAtt                 <  1:245890/81‑1 (MQ=255)                                                          aaCCCATGTCAAAGTCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGt            >  1:245914/1‑81 (MQ=255)                                                           aCCCATGTCAAAGTCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTc           >  2:245917/1‑81 (MQ=255)                                                              cATGTCAAAGTCGCTCAGCTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGCCtt         >  1:245912/1‑80 (MQ=255)                                                                    aaaGTCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTActct   <  1:245894/80‑1 (MQ=255)                                                                    aaaGTCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTActct   <  2:245891/80‑1 (MQ=255)                                                                    aaaGTCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTActct   <  2:245893/80‑1 (MQ=255)                                                                    aaaGTCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTg  <  2:245892/81‑1 (MQ=255)                                                                        |                                                                             CATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTG  >  NC_000913/1253744‑1253891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑