Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,277,223	G→C	A132A (GCC→GCG)	narX ←	sensory histidine kinase NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,277,223	0	G	C	100.0%	81.0 / NA	24	A132A (GCC→GCG)	narX	sensory histidine kinase NarX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (11/13);  total (11/13)

GATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATTACG  >  NC_000913/1277150‑1277300                                                                          |                                                                              gATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGcaaca                                                                         >  1:249592/1‑80 (MQ=255)    ggACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACt                                                                     >  2:249575/1‑81 (MQ=255)          gTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGa                                                               <  1:249530/81‑1 (MQ=255)           tACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGa                                                               >  2:249573/1‑80 (MQ=255)                   tctcGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCt                                                       <  1:249531/80‑1 (MQ=255)                    ctcGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGa                                                     >  2:249570/1‑81 (MQ=255)                        aTGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACAcc                                                 <  1:249532/81‑1 (MQ=255)                        aTGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACAcc                                                 <  2:249533/81‑1 (MQ=255)                             cATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTtc                                            <  1:249534/81‑1 (MQ=255)                                     tggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGtttt                                    <  2:249535/81‑1 (MQ=255)                                         gCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTtgtg                                 >  2:249582/1‑80 (MQ=255)                                          cGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCa                               >  2:249577/1‑81 (MQ=255)                                             tCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGc                            <  2:249536/81‑1 (MQ=255)                                             tCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGc                            <  1:249537/81‑1 (MQ=255)                                               aaaaCCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCAt                          >  2:249576/1‑81 (MQ=255)                                                     aGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAgcg                     >  1:249580/1‑80 (MQ=255)                                                       aTACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAg                  <  2:249538/81‑1 (MQ=255)                                                         aCCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAgg                 >  1:249574/1‑80 (MQ=255)                                                          ccAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGa               <  1:249539/81‑1 (MQ=255)                                                           cAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGAt              <  2:249540/81‑1 (MQ=255)                                                           cAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGAt              >  2:249578/1‑81 (MQ=255)                                                                 aTCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGtt         >  2:249588/1‑80 (MQ=255)                                                                    aaGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCAt      <  1:249541/80‑1 (MQ=255)                                                                      gCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATTAc   <  2:249542/81‑1 (MQ=255)                                                                       ccccGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATTACg  >  1:249586/1‑81 (MQ=255)                                                                          |                                                                              GATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATTACG  >  NC_000913/1277150‑1277300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑