Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,284,681	T→C	S360P (TCT→CCT)	narH →	nitrate reductase A subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,284,681	0	T	C	100.0%	87.3 / NA	26	S360P (TCT→CCT)	narH	nitrate reductase A subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/13);  total (13/13)

AATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATT  >  NC_000913/1284605‑1284758                                                                             |                                                                              aatggaatggAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTcc                                                                           <  2:250747/81‑1 (MQ=255)     gaatggaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGAtt                                                                       >  1:250777/1‑81 (MQ=255)     gaatggaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGAt                                                                        <  2:250748/80‑1 (MQ=255)            aaatGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTg                                                                <  2:250749/78‑1 (MQ=255)             aCTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTgc                                                               <  1:250750/81‑1 (MQ=255)               tGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTgcag                                                             >  1:250782/1‑81 (MQ=255)                  cgctgccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGcagcag                                                          <  2:250751/81‑1 (MQ=255)                   gctgccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGa                                                         >  2:250779/1‑81 (MQ=255)                       ccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGAcgc                                                      <  2:250752/80‑1 (MQ=255)                           tgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGt                                                  >  1:250791/1‑80 (MQ=255)                            gcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGt                                                  >  2:250784/1‑79 (MQ=255)                                 cGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTgg                                           >  2:250788/1‑81 (MQ=255)                                  ggAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTggg                                          >  1:250785/1‑81 (MQ=255)                                     atatCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAg                                       <  1:250753/81‑1 (MQ=255)                                      tatCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAg                                       >  2:250786/1‑80 (MQ=255)                                        tCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCaa                                    <  2:250754/81‑1 (MQ=255)                                           cacaCTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACgg                                 >  1:250794/1‑81 (MQ=255)                                                      tGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAg                      <  2:250755/81‑1 (MQ=255)                                                       ggTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGa                     >  2:250847/1‑81 (MQ=255)                                                        gTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGa                     <  2:250756/80‑1 (MQ=255)                                                         tCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACg                   <  2:250757/81‑1 (MQ=255)                                                         tCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACg                   <  1:250758/81‑1 (MQ=255)                                                             gTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCg                >  2:250806/1‑80 (MQ=255)                                                              tACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGa               >  1:250810/1‑80 (MQ=255)                                                                     cgccTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGc       <  2:250759/81‑1 (MQ=255)                                                                          ctGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTAtt  >  2:250807/1‑81 (MQ=255)                                                                             |                                                                              AATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATT  >  NC_000913/1284605‑1284758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑