Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,296,554	C→T	S523N (AGC→AAC)	adhE ←	aldehyde‑alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,296,554	0	C	T	100.0%	114.8 / NA	32	S523N (AGC→AAC)	adhE	aldehyde‑alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/18);  total (14/18)

TCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCTTTCAGTACGGAAGTGAT  >  NC_000913/1296477‑1296627                                                                              |                                                                          tCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCa                                                                        <  2:252512/81‑1 (MQ=255)     ggAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAgggt                                                                    <  1:252513/81‑1 (MQ=255)     ggAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAgggt                                                                    >  1:252546/1‑81 (MQ=255)      gAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAgggtc                                                                   <  2:252514/81‑1 (MQ=255)       aaCCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAgggtcg                                                                  <  1:252515/81‑1 (MQ=255)        accaccaccCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAgggtcgg                                                                 <  2:252516/81‑1 (MQ=255)             caccCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCg                                                            >  1:252550/1‑81 (MQ=255)               cccAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCt                                                          <  1:252517/81‑1 (MQ=255)                  aGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTc                                                        >  2:252545/1‑80 (MQ=255)                     gcgATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACt                                                    >  1:252549/1‑81 (MQ=255)                       gATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTc                                                  >  1:252554/1‑81 (MQ=255)                         tAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCga                                                <  1:252518/81‑1 (MQ=255)                              aCGTCTGGTTTGAAGGAGTTTGCCAGTTCTTCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCgaag                                              >  1:252557/1‑78 (MQ=255)                                gTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGAc                                         <  1:252519/81‑1 (MQ=255)                                     gTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACtt                                       <  1:252520/78‑1 (MQ=255)                                        tGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGtt                                  <  1:252521/80‑1 (MQ=255)                                         gAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGttt                                 <  2:252522/80‑1 (MQ=255)                                          aaGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTc                                <  2:252523/80‑1 (MQ=255)                                          aaGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTc                                <  1:252524/80‑1 (MQ=255)                                              aGTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGc                           >  1:252555/1‑81 (MQ=255)                                               gTTTGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGcc                          <  1:252525/81‑1 (MQ=255)                                                tttGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCct                         >  1:252556/1‑81 (MQ=255)                                                  tGCCAGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACGTCGAAGAAGACTTCAGTTTCAACGCctg                        <  2:252526/80‑1 (MQ=255)                                                      aGTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTgctgct                   <  2:252527/81‑1 (MQ=255)                                                       gTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCtt                  >  1:252560/1‑81 (MQ=255)                                                       gTTCTGCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCtt                  >  1:252541/1‑81 (MQ=255)                                                            gCACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCTTTCAg              >  1:252567/1‑80 (MQ=255)                                                             cACCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCTTTCa               <  1:252528/78‑1 (MQ=255)                                                              aCCTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCTTTCAGTAc           >  1:252551/1‑81 (MQ=255)                                                               ccTTTACGAACGATGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCTTTCAGTACg          <  1:252529/81‑1 (MQ=255)                                                                       aacgaTGTTCAGGGTCGGGTCCGCTTCTACTTCGTAGAAGACTTCAGTTTCAACGCCTGCTGCTTTCAGTACGGAAGtgat  >  1:252559/1‑81 (MQ=255)                                                                       aacgaTGTTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCTTTCAGTACGGAAGtgat  >  1:252562/1‑81 (MQ=255)                                                                              |                                                                          TCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCTCAGGGTCGGGTCCGCTTCTACTTCGAAGAAGACTTCAGTTTCAACGCCTGCTGCTTTCAGTACGGAAGTGAT  >  NC_000913/1296477‑1296627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑