Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,299,534	T→C	F2L (TTT→CTT)	insH21 →	IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,299,534	0	T	C	100.0%	38.4 / NA	13	F2L (TTT→CTT)	insH21	IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/7);  total (6/7)

TATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATG  >  NC_000913/1299459‑1299569                                                                            |                                    tattatTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTc                                <  2:252919/81‑1 (MQ=255)        aaTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTg                           >  2:252942/1‑79 (MQ=255)         aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAg                        <  1:252920/81‑1 (MQ=255)             cACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCa                     >  2:252948/1‑80 (MQ=255)               cTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCa                     >  1:252944/1‑78 (MQ=255)                  ccTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAAc               <  1:252921/81‑1 (MQ=255)                    tttGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAg             >  2:252949/1‑81 (MQ=255)                      tGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAgg            <  2:252922/80‑1 (MQ=255)                       ggTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAggg           <  2:252923/80‑1 (MQ=255)                         tAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGTtc        <  2:252924/81‑1 (MQ=255)                          agagGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGTtca       >  2:252953/1‑81 (MQ=255)                               aaaGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGTTCATCATg  <  1:252925/81‑1 (MQ=25)                                aaGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGTTcatcat   >  1:252987/1‑79 (MQ=21)                                                                            |                                    TATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATG  >  NC_000913/1299459‑1299569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑