Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,308,572	A→G	L25S (TTA→TCA)	clsA ←	cardiolipin synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,308,572	0	A	G	100.0%	89.6 / NA	26	L25S (TTA→TCA)	clsA	cardiolipin synthase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/10);  total (16/10)

CGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGAT  >  NC_000913/1308495‑1308649                                                                              |                                                                              cGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAg                                                                            >  1:254321/1‑81 (MQ=255)  gACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGt                                                                           >  1:254315/1‑81 (MQ=255)   aCTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGtt                                                                          <  1:254291/81‑1 (MQ=255)    cTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTa                                                                         >  2:254320/1‑81 (MQ=255)     tAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTAc                                                                        >  2:254318/1‑81 (MQ=255)       aCGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGc                                                                      <  1:254292/81‑1 (MQ=255)         ggCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCt                                                                    >  2:254299/1‑81 (MQ=255)                    aataatCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCaa                                                         >  1:254328/1‑81 (MQ=255)                          cAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTa                                                   >  2:254319/1‑81 (MQ=255)                             cAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATcc                                                >  1:254339/1‑81 (MQ=255)                              aGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATcc                                                >  1:254323/1‑80 (MQ=255)                                      cATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGc                                       >  1:254342/1‑81 (MQ=255)                                       aTCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGc                                       >  1:254349/1‑80 (MQ=255)                                            ggAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGccaacca                                 <  1:254293/81‑1 (MQ=255)                                             gAGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGccaaccaa                                <  1:254294/81‑1 (MQ=255)                                              aGGGAACTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCcaaccaac                               <  2:254295/81‑1 (MQ=255)                                                   aCTGCGCGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCAcc                          <  1:254296/81‑1 (MQ=255)                                                      gcgcGTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAAc                       >  1:254338/1‑81 (MQ=255)                                                       cgcgTCGTTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACg                      <  1:254297/81‑1 (MQ=255)                                                          gtcgtTTCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGta                    >  1:254314/1‑80 (MQ=255)                                                               ttCATTAGAATGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACg              >  2:254330/1‑81 (MQ=255)                                                                  aTTAGAATGTGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGtt           <  1:254298/81‑1 (MQ=255)                                                                       aaTGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCAt      <  1:254299/81‑1 (MQ=255)                                                                         tGCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAg    <  2:254300/81‑1 (MQ=255)                                                                          gCGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCa       >  1:254324/1‑77 (MQ=255)                                                                           cGTGAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGAt  >  2:254326/1‑81 (MQ=255)                                                                              |                                                                              CGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGAT  >  NC_000913/1308495‑1308649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑