Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,313,464	A→G	V67A (GTT→GCT)	yciC ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,313,464	0	A	G	100.0%	41.2 / NA	13	V67A (GTT→GCT)	yciC	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/6);  total (7/6)

CGATTAATCCTGAAAAAGTGGACGCCGCTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGT  >  NC_000913/1313390‑1313526                                                                           |                                                               cGATTAATCCTGAAAAAGTGGACGCCGCTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGt                                                          >  1:254934/1‑81 (MQ=255)  gATTTATCCTGAAAAAGTTGACGCCGCTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTc                                                         <  2:254908/81‑1 (MQ=255)       aTCCTGAAAAAGTGGACGCCGCTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGaac                                                     <  2:254909/80‑1 (MQ=255)             aaaaaGTGGACGCCGCTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCac                                              >  1:254939/1‑81 (MQ=255)                          cgcTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAAc                                 <  2:254910/81‑1 (MQ=255)                            cTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACgg                               >  1:254940/1‑81 (MQ=255)                              gAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACggg                              >  1:254937/1‑80 (MQ=255)                                aGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCAc                           >  1:254951/1‑81 (MQ=255)                                    tGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCg                       >  2:254935/1‑81 (MQ=255)                                             aTTTGTTGCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGc              >  1:254936/1‑81 (MQ=255)                                                 gttgCTGTTCCGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTgc           <  1:254911/80‑1 (MQ=255)                                                          ccGGTGACATATTTTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCtgt  <  1:254913/80‑1 (MQ=255)                                                          ccGGTGACATATTCTGAGCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCtgt  <  1:254912/80‑1 (MQ=255)                                                                           |                                                               CGATTAATCCTGAAAAAGTGGACGCCGCTGAAGCCTGCAGCAAAATTTGTTGCTGTTCCGGTGACATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGT  >  NC_000913/1313390‑1313526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑