Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,320,045	G→T	A447E (GCA→GAA)	trpD ←	anthranilate synthase subunit TrpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,320,045	0	G	T	100.0%	109.1 / NA	31	A447E (GCA→GAA)	trpD	anthranilate synthase subunit TrpD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/18);  total (13/18)

GTTAAAATGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGGCGCGTGTAATGAAACTTCATC  >  NC_000913/1319967‑1320121                                                                               |                                                                             gtTAAAATGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCg                                                                            >  2:255890/1‑81 (MQ=255) gtTAAAATGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCg                                                                            >  1:255889/1‑81 (MQ=255)  ttAAAATGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCgg                                                                           >  2:255901/1‑81 (MQ=255)  ttAAAATGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCgg                                                                           >  2:255891/1‑81 (MQ=255)  ttAAAATGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCg                                                                            <  2:255856/80‑1 (MQ=255)       aTGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGc                                                                      <  1:255857/81‑1 (MQ=255)               gTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCGGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCt                                                              <  2:255858/81‑1 (MQ=255)                ttttCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCtt                                                             <  1:255859/81‑1 (MQ=255)                   tcttcCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCtttt                                                           <  1:255860/80‑1 (MQ=255)                       ccGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAAt                                                        <  2:255861/79‑1 (MQ=255)                        cGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTc                                                     >  1:255897/1‑81 (MQ=255)                              tCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGt                                                >  2:255900/1‑80 (MQ=255)                               ccGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTc                                               <  2:255863/80‑1 (MQ=255)                               ccGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTc                                               <  2:255862/80‑1 (MQ=255)                                  ccTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATg                                            >  1:255898/1‑80 (MQ=255)                                  ccTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGc                                           <  2:255864/81‑1 (MQ=255)                                     gCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGt                                        >  2:255905/1‑81 (MQ=255)                                      ccAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAg                                         <  2:255865/79‑1 (MQ=255)                                        aGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCg                                     >  1:255909/1‑81 (MQ=255)                                                cTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACg                               <  2:255867/79‑1 (MQ=255)                                                cTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGa                              <  2:255866/80‑1 (MQ=255)                                                   gtggtAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGAtt                            <  1:255869/79‑1 (MQ=255)                                                   gtggtAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGAtt                            <  1:255868/79‑1 (MQ=255)                                                      gtAGGGAGTCAGGCCAAAGTCTTCTTCGATGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCgg                       <  1:255870/81‑1 (MQ=255)                                                       tAGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGgc                      <  2:255871/81‑1 (MQ=255)                                                        aGGGTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGgcg                     >  2:255907/1‑81 (MQ=255)                                                         gggTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGgcgc                    <  1:255872/81‑1 (MQ=255)                                                          ggTGTCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGGcgcg                   >  1:255904/1‑81 (MQ=255)                                                            tgtCAGGCCAAAGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGGCGCgtg                 <  2:255873/81‑1 (MQ=255)                                                                     aaaGTCTTCTTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGGCGCGTGTAATGAAAc        >  1:255902/1‑81 (MQ=255)                                                                            tctTCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGGCGCGTGTAATGAAACTTcatc  >  1:255903/1‑80 (MQ=255)                                                                               |                                                                             GTTAAAATGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTTCGGCAACGATTGTCGGCGCGTGTAATGAAACTTCATC  >  NC_000913/1319967‑1320121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑