Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	150,833	A→G	S41P (TCC→CCC)	yadC ←	fimbrial tip‑adhesin YadC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	150,833	0	A	G	100.0%	67.2 / NA	20	S41P (TCC→CCC)	yadC	fimbrial tip‑adhesin YadC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/5);  total (15/5)

GGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGT‑GGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACA  >  NC_000913/150755‑150908                                                                                |                                                                            ggCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGT‑Ggggg                                                                           >  2:36382/1‑81 (MQ=255)     gAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGT‑GGGGGGGcc                                                                       >  1:36396/1‑81 (MQ=255)        cTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGT‑GGGGGGGCCTc                                                                     >  1:36398/1‑80 (MQ=255)             tGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGT‑GGGGGGGCCTCGATAGt                                                               <  1:36374/81‑1 (MQ=255)             tGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGT‑GGGGGGGCCTCGATAGt                                                               <  2:36375/81‑1 (MQ=255)              gggTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGTGGGGGGGGCCTCGATAGt                                                               >  1:36392/1‑81 (MQ=255)                            ccAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCat                                                >  1:36400/1‑81 (MQ=255)                                 ttgcttgcTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCATTAttt                                           >  2:36397/1‑81 (MQ=255)                                    cttgctGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCATTATTTccc                                        <  1:36376/81‑1 (MQ=255)                                      tgctGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCca                                       >  1:36395/1‑80 (MQ=255)                                          gTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAAt                                   >  2:36401/1‑80 (MQ=255)                                             gATGTGTAATTAAAGGTCATCTGTGCGGCAGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATaaaa                               >  1:36402/1‑81 (MQ=255)                                               tgtgTAATTAAAGGTCATCTGTGCGGCAGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACt                             >  2:36403/1‑81 (MQ=255)                                                    aaTTAAAGGTCATCTGTGCGGCAGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGta                         >  2:36406/1‑80 (MQ=255)                                                       tAAAGGTCATCTGTGCGGCAGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATg                     >  1:36407/1‑81 (MQ=255)                                                        aaaGGTCATCTGTGCGGCAGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATg                     >  1:36405/1‑80 (MQ=255)                                                         aaGGTCATCTGTGCGGCAGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGc                    <  2:36377/80‑1 (MQ=255)                                                           ggTCATCTGTGCGGCAGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCact                 >  2:36399/1‑81 (MQ=255)                                                                  tgtgCGGCAGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTa          >  1:36409/1‑81 (MQ=255)                                                                      cGGCAGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTa       >  2:36404/1‑80 (MQ=255)                                                                          aGT‑GGGGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTacaaca  <  1:36378/81‑1 (MQ=255)                                                                                |                                                                            GGCCGAGCTGCGTGGGTTTATAAAAAACCAAGTTGCTTGCTGTTGATGTGTAATTAAAGGTCATCTGTGCGGCAGT‑GGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACA  >  NC_000913/150755‑150908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑