Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,322,638	C→A	L103L (CTG→CTT)	trpE ←	anthranilate synthase subunit TrpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,322,638	0	C	A	100.0%	72.0 / NA	21	L103L (CTG→CTT)	trpE	anthranilate synthase subunit TrpE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/6);  total (15/6)

TAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCC  >  NC_000913/1322560‑1322704                                                                               |                                                                   tAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCgaag                                                                  >  2:256336/1‑81 (MQ=255)  aaaCGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCgaag                                                                  >  1:256345/1‑80 (MQ=255)  aaaCGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCgaag                                                                  >  2:256350/1‑80 (MQ=255)         aaGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGc                                                          <  2:256304/81‑1 (MQ=255)             gTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGtt                                                      >  1:256348/1‑81 (MQ=255)             gTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCAAAGCACACGGCAGtt                                                      <  1:256305/81‑1 (MQ=255)                    aCCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGAt                                               >  2:256333/1‑81 (MQ=255)                       gAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGt                                            >  1:256334/1‑81 (MQ=255)                          aGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCa                                         >  1:256331/1‑81 (MQ=255)                             gAGCATAAGCGGGCGTCTTCATACAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACtt                                      >  2:256352/1‑81 (MQ=255)                              aGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACttt                                     <  1:256306/81‑1 (MQ=255)                                 aTAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACTTTCca                                  >  2:256343/1‑81 (MQ=255)                                 aTAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACTTTCca                                  >  1:256349/1‑81 (MQ=255)                                       gggCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCg                            <  2:256307/81‑1 (MQ=255)                                           gTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCgcagg                        >  1:256353/1‑81 (MQ=255)                                            tCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCgcagg                        >  2:256344/1‑80 (MQ=255)                                             cTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCgcagg                        >  2:256358/1‑79 (MQ=255)                                             cTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCgcagg                        >  2:256356/1‑79 (MQ=255)                                             cTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCgcagg                        >  2:256346/1‑79 (MQ=255)                                                    cagcagTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGt                >  2:256347/1‑80 (MQ=255)                                                      gcagTGGACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTAt             >  1:256325/1‑81 (MQ=255)                                                           ggACTGACAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCag         >  2:256342/1‑80 (MQ=255)                                                                 aCAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGcc  <  2:256308/81‑1 (MQ=255)                                                                 aCAGGGGGGAAGCGAAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGc   <  1:256309/80‑1 (MQ=255)                                                                               |                                                                   TAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCC  >  NC_000913/1322560‑1322704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑