Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,323,360	C→T	T47T (ACC→ACT)	yciV →	RNA/ssDNA 5'‑>3' exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,323,360	0	C	T	100.0%	57.7 / NA	17	T47T (ACC→ACT)	yciV	RNA/ssDNA 5'‑>3' exonuclease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/8);  total (9/8)

CTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACCACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTCCCG  >  NC_000913/1323286‑1323427                                                                           |                                                                    cTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCt                                                               <  2:256416/81‑1 (MQ=255)      gCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAAt                                                          <  2:256417/81‑1 (MQ=255)         aGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGTTCACCGACCATGACACTACAGCTGCAATcgc                                                       >  2:256449/1‑81 (MQ=255)            aGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGcc                                                    <  2:256418/81‑1 (MQ=255)             gCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCa                                                   <  2:256419/81‑1 (MQ=255)               aTTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAg                                                  >  1:256452/1‑80 (MQ=255)                             cAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAAttt                                   <  1:256420/81‑1 (MQ=255)                               gTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCa                                 <  2:256421/81‑1 (MQ=255)                                 cGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACg                               >  2:256440/1‑81 (MQ=255)                                      tGCGCGTCGGCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCgg                          >  1:256446/1‑81 (MQ=255)                                              ggCACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGc                   <  2:256422/80‑1 (MQ=255)                                                cACCCTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGtt                >  2:256447/1‑81 (MQ=255)                                                  cccTGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTg               >  1:256454/1‑80 (MQ=255)                                                     tGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATc           >  1:256455/1‑81 (MQ=255)                                                     tGGCGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATc           >  1:256457/1‑81 (MQ=255)                                                        cGATCACCGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCTTa        >  2:256451/1‑81 (MQ=255)                                                              ccGACCATGACACTACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTCCCg  <  1:256423/81‑1 (MQ=255)                                                                           |                                                                    CTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGCACCCTGGCGATCACCGACCATGACACCACAGCTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTCCCG  >  NC_000913/1323286‑1323427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑