Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,323,906	C→G	C229W (TGC→TGG)	yciV →	RNA/ssDNA 5'‑>3' exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,323,906	0	C	G	100.0%	88.1 / NA	26	C229W (TGC→TGG)	yciV	RNA/ssDNA 5'‑>3' exonuclease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (15/11);  total (15/11)

CTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGA  >  NC_000913/1323836‑1323983                                                                       |                                                                              cTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGc                                                                     <  1:256492/81‑1 (MQ=255)  tAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGcc                                                                    >  1:256527/1‑81 (MQ=255)        gCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATg                                                               <  2:256493/80‑1 (MQ=255)            aaaaGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAAc                                                            <  2:256494/79‑1 (MQ=255)            aaaaGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACg                                                           >  2:256524/1‑80 (MQ=255)             aaaGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTa                                                         <  2:256495/81‑1 (MQ=255)              aaGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTAc                                                        >  1:256528/1‑81 (MQ=255)               aGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTAcc                                                       >  1:256540/1‑81 (MQ=255)                       tGCGCATTTTGACGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTACTCAGCTg                                                 >  1:256530/1‑79 (MQ=255)                         cgcATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTggcgg                                             >  2:256522/1‑81 (MQ=255)                             ttttGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCt                                         >  2:256535/1‑81 (MQ=255)                             ttttGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCt                                         <  2:256496/81‑1 (MQ=255)                              tttGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCtt                                        >  1:256523/1‑81 (MQ=255)                              tttGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCtt                                        >  2:256532/1‑81 (MQ=255)                                tGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTgc                                      >  1:256542/1‑81 (MQ=255)                                     aaCACCACGGTGACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTc                                 <  2:256497/81‑1 (MQ=255)                                                gACGCGATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTa                      >  2:256525/1‑81 (MQ=255)                                                   gcgATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATgg                   >  1:256536/1‑81 (MQ=255)                                                     gATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATggg                  >  1:256529/1‑80 (MQ=255)                                                     gATGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGc                 <  2:256498/81‑1 (MQ=255)                                                      aTGGAAGTCGCGCAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCa                >  2:256539/1‑81 (MQ=255)                                                               gcgcAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGa       <  1:256499/81‑1 (MQ=255)                                                                cgcAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGAt      <  2:256500/81‑1 (MQ=255)                                                                cgcAGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGAt      >  2:256533/1‑81 (MQ=255)                                                                   aGTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTg   <  2:256501/81‑1 (MQ=255)                                                                    gTGGCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGa  <  1:256502/81‑1 (MQ=255)                                                                       |                                                                              CTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGA  >  NC_000913/1323836‑1323983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑