Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,333,096	T→C	C683C (TGT→TGC)	topA →	DNA topoisomerase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,333,096	0	T	C	100.0%	99.5 / NA	29	C683C (TGT→TGC)	topA	DNA topoisomerase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/16);  total (13/16)

TCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGT  >  NC_000913/1333027‑1333173                                                                      |                                                                              tcgtTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAAccc                                                                    <  1:257911/81‑1 (MQ=255)   gtTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCaa                                                                  >  1:257953/1‑81 (MQ=255)        ccGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGc                                                             <  1:257912/81‑1 (MQ=255)        ccGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGc                                                             >  1:257948/1‑81 (MQ=255)           aaaTGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGAc                                                          <  2:257913/81‑1 (MQ=255)            aaTGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACg                                                         <  1:257914/81‑1 (MQ=255)               gCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGt                                                       >  1:257956/1‑80 (MQ=255)                cGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGtt                                                      <  2:257915/80‑1 (MQ=255)                cGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGtt                                                      <  2:257916/80‑1 (MQ=255)                 ggCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTAc                                                    >  1:257951/1‑81 (MQ=255)                       gCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATc                                              <  2:257917/81‑1 (MQ=255)                        cGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCg                                             <  2:257918/81‑1 (MQ=255)                            ggACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGaa                                           >  1:257949/1‑79 (MQ=255)                              aCAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAgg                                       <  2:257919/81‑1 (MQ=255)                                aGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGc                                     <  1:257920/81‑1 (MQ=255)                                 gCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCg                                    <  2:257921/81‑1 (MQ=255)                                  cTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCg                                    >  2:257961/1‑80 (MQ=255)                                          tCGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCa                           <  2:257922/81‑1 (MQ=255)                                           cGATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCAt                          >  1:257964/1‑81 (MQ=255)                                            gATCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCAt                          <  1:257923/80‑1 (MQ=255)                                              tCCGAAACGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTa                        >  2:257954/1‑80 (MQ=255)                                                  aaaCGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGt                   >  2:257958/1‑81 (MQ=255)                                                   aaCGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGt                   >  1:257959/1‑80 (MQ=255)                                                     cGTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTAt                <  1:257924/81‑1 (MQ=255)                                                      gTAAGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATg               <  2:257925/81‑1 (MQ=255)                                                        aaGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGAc             >  2:257947/1‑81 (MQ=255)                                                         aGTTGCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACg            >  1:698270/1‑81 (MQ=255)                                                             gCATGTCTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGcc         <  1:257926/80‑1 (MQ=255)                                                                   cTGCGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGt  >  2:257957/1‑81 (MQ=255)                                                                      |                                                                              TCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGT  >  NC_000913/1333027‑1333173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑