Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,347,878	T→C	Q345Q (CAA→CAG)	rnb ←	RNase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,347,878	0	T	C	100.0%	72.6 / NA	22	Q345Q (CAA→CAG)	rnb	RNase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/10);  total (12/10)

TTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTC  >  NC_000913/1347802‑1347952                                                                             |                                                                           tttCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTg                                                                          <  2:260072/79‑1 (MQ=255)   tCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCa                                                                       <  1:260073/80‑1 (MQ=255)     aCCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCaaa                                                                     <  2:260074/80‑1 (MQ=255)        gagaATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTg                                                                 >  2:260102/1‑81 (MQ=255)         agaATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTgc                                                                <  1:260075/81‑1 (MQ=255)                  cGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCaaa                                                       >  2:260114/1‑81 (MQ=255)                      aaTCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCAAACGGa                                                   >  2:260117/1‑81 (MQ=255)                       aTCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCAAACGGAc                                                  >  2:260100/1‑81 (MQ=255)                             gCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCAAACGGACTTGTTc                                            >  2:260110/1‑81 (MQ=255)                             gCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCAAACGGACTTGTTc                                            <  1:260076/81‑1 (MQ=255)                              cGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCAAACGGACTTGTTCg                                           <  1:260077/81‑1 (MQ=255)                                 tCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGc                                         >  2:260106/1‑80 (MQ=255)                                     tAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAAtt                                     <  2:260078/80‑1 (MQ=255)                                       aaCACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGc                                   <  1:260079/80‑1 (MQ=255)                                       aaCACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCt                                  >  2:260107/1‑81 (MQ=255)                                        acacCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCtt                                 >  2:260115/1‑81 (MQ=255)                                           ccAGTGCGTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCAc                              <  2:260080/81‑1 (MQ=255)                                                  gTGGTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAgg                       >  1:260112/1‑81 (MQ=255)                                                    ggTTATGACGCCACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCt                     >  2:260101/1‑81 (MQ=255)                                                               cACTCGCCGCGGCGCTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCg          <  2:260081/81‑1 (MQ=255)                                                                   cgccgcGGCGCTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTAt      >  1:260111/1‑81 (MQ=255)                                                                        cggcgCTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTc  >  1:260108/1‑80 (MQ=255)                                                                             |                                                                           TTTCACCGAGAATAAAGCGGTAATCCGGGCGATCTTTAAACACCAGTGCGTGGTTATGACGCCACTCGCCGCGGCGTTGGCAAATTTGCGCTAGCAAACGGACTTGTTCGGCAATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTC  >  NC_000913/1347802‑1347952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑