Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,481	G→C	P13R (CCG→CGG)	sapC ←	putrescine ABC exporter membrane protein SapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,481	0	G	C	100.0%	81.5 / NA	24	P13R (CCG→CGG)	sapC	putrescine ABC exporter membrane protein SapC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (10/14);  total (10/14)

CCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCA  >  NC_000913/1354407‑1354557                                                                           |                                                                             ccGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCcgcgggc                                                                        <  2:261148/81‑1 (MQ=255)    cgcAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCCGCGGGCGCt                                                                     <  2:261149/81‑1 (MQ=255)     gcAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCCGCGGGCGCtt                                                                    <  1:261150/81‑1 (MQ=255)       aGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCCGCGGGCGCtttt                                                                  >  1:261182/1‑81 (MQ=255)            tACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCCGCGGGCGCTTTTCGCTg                                                             <  2:261151/81‑1 (MQ=255)              cAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCCGCGGGCGCTTTTCGCTGt                                                            <  2:261153/80‑1 (MQ=255)              cAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCAGTACGCAGCGTGCCCCGCGGGCGCTTTTCGCTGt                                                            <  1:261152/80‑1 (MQ=255)                  ccGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACg                                                       <  2:261154/81‑1 (MQ=255)                    gACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACGc                                                      >  2:261194/1‑80 (MQ=255)                    gACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACGc                                                      >  1:261184/1‑80 (MQ=255)                        aTCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACGCTATCg                                                 >  1:261186/1‑81 (MQ=255)                              gagGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACGCTATCGTAAgg                                            >  1:261176/1‑80 (MQ=255)                              gagGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGc                                           <  1:261155/81‑1 (MQ=255)                                ggCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCAt                                         <  1:261156/81‑1 (MQ=255)                                          taAAATTTGCGCCAGGCGGTACGCAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCt                                <  1:261157/80‑1 (MQ=255)                                                 tGCGCCAGGCGGTACGCAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATTCCATTCCTTATGttt                         <  1:261158/80‑1 (MQ=255)                                                           ggTACGCAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAg              >  1:261187/1‑81 (MQ=255)                                                             tACGCAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGcc            <  2:261159/81‑1 (MQ=255)                                                             tACGCAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGc             >  2:261197/1‑80 (MQ=255)                                                               cGCAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCAt          <  2:261160/81‑1 (MQ=255)                                                               cGCAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCAt          <  1:261161/81‑1 (MQ=255)                                                                 cAGCGTGCCCCGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATgg        >  1:261190/1‑81 (MQ=255)                                                                     gTGCCCCGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCAcc    >  1:261193/1‑81 (MQ=255)                                                                      tGCCCCGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCAccc   <  1:261162/81‑1 (MQ=255)                                                                       gCCCCGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCAccc   >  2:261188/1‑80 (MQ=255)                                                                        ccccGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCa  <  1:261163/80‑1 (MQ=255)                                                                           |                                                                             CCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCA  >  NC_000913/1354407‑1354557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑