Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,373,895	C→T	T225I (ACA→ATA)	ycjO →	putative ABC transporter membrane subunit YcjO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,373,895	0	C	T	100.0%	104.5 / NA	30	T225I (ACA→ATA)	ycjO	putative ABC transporter membrane subunit YcjO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/11);  total (19/11)

GATGGCGCTAATGCCTGGCAACGGTTTCGTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGACACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTATTTGCTGACGACCAAGGTCGATATTCTCGGTG  >  NC_000913/1373819‑1373969                                                                             |                                                                           gatggCGCTAATGCCTGGCAACGGTTTCGTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACt                                                                         <  1:264200/80‑1 (MQ=255) gatggCGCTAATGCCTGGCAACGGTTTCGTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTg                                                                        >  2:264263/1‑81 (MQ=255)    ggCGCTAATGCCTGGCAACGGTTTCGTATCGTCACGCTGCCCGCAATTACGCCGGTCCTGGCGACGGTGGTGATACTgcg                                                                      >  2:264228/1‑80 (MQ=255)      cgcTAATGCCTGGCAACGGTTTCGTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCAc                                                                   >  2:264232/1‑81 (MQ=255)       gcTAATGCCTGGCAACGGTTTCGTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCAc                                                                   >  1:264231/1‑80 (MQ=255)          aaTGCCTGGCAACGGTTTCGTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATc                                                               >  2:264238/1‑81 (MQ=255)             gCCTGGCAACGGTTTCGTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTg                                                             >  1:264243/1‑80 (MQ=255)               cTGGCAACGGTTTCGTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGAt                                                          <  1:264201/81‑1 (MQ=255)                  gCAACGGTTTCGTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGtt                                                       >  1:264250/1‑81 (MQ=255)                  gCAACGGTTTCGTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGtt                                                       >  1:264252/1‑81 (MQ=255)                   cAACGGTTTCGTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTc                                                      <  2:264202/81‑1 (MQ=255)                    aaCGGTTTCGTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTc                                                      >  1:264242/1‑80 (MQ=255)                       ggTTTCGTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTCTACa                                                  >  2:264226/1‑81 (MQ=255)                             gTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTcg                                            <  1:264203/81‑1 (MQ=255)                                      cGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGttt                                   <  2:264204/81‑1 (MQ=255)                                        cTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTAt                                 <  1:264205/81‑1 (MQ=255)                                         tGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTAtt                                >  2:264244/1‑81 (MQ=255)                                           cccGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTATTTg                              <  2:264206/81‑1 (MQ=255)                                           cccGCAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTATTTg                              <  2:264207/81‑1 (MQ=255)                                               cAATTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTATTTGCTga                          >  2:264241/1‑81 (MQ=255)                                                aaTTATGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTATTTGCTgac                         >  2:264254/1‑81 (MQ=255)                                                     tGCCGGTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTATTTGCTGACGAcc                     <  2:264208/80‑1 (MQ=255)                                                         ggTCCTGGCGACGGTGGTGATGCTGCGCACCATCTAAATGTTCTGCATGTTCACGGATGTTTATTTGCTGACGACCAAGGt                <  2:1028782/81‑1 (MQ=255)                                                         ggTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTATTTGCTGACGACCAAGGt                <  2:264209/81‑1 (MQ=255)                                                          gTCCTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTATTTGCTGACGACCAAGGTc               >  2:264249/1‑81 (MQ=255)                                                             cTGGCGACGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTATTTGCTGACGACCAAGGTCGa             >  2:264253/1‑80 (MQ=255)                                                                 cGACGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTATTTGCTGACGACCAAGGTCGATATtc        >  2:264255/1‑81 (MQ=255)                                                                  gACGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTATTTGCTGACGACCAAGGTCGATATtct       >  2:264256/1‑81 (MQ=255)                                                                    cGGTGGTGATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTATTTGCTGACGACCAAGGTCGATATTCTCg     >  1:264245/1‑81 (MQ=255)                                                                        ggtgATACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTATTTGCTGACGACCAAGGTCGATATTCTCGgtg  >  2:264273/1‑80 (MQ=255)                                                                             |                                                                           GATGGCGCTAATGCCTGGCAACGGTTTCGTATCGTCACGCTGCCCGCAATTATGCCGGTCCTGGCGACGGTGGTGACACTGCGCACCATCTGGATGTTCTACATGTTCGCGGATGTTTATTTGCTGACGACCAAGGTCGATATTCTCGGTG  >  NC_000913/1373819‑1373969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑